L5162

Lymphotoxin-α1/β2 human

≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder

• Synonym(s): LT-α, LT-β, TNF-β, Tumor Necrosis Factor-β
• MDL number: MFCD00163958
• UNSPSC Code: 12352202

Properties

• biological source: human
• recombinant: expressed in baculovirus infected Sf21 cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.02-0.10 μg/mL ED₅₀
• mol wt: subunit calculated mol wt ~18 kDa (LT- alpha); subunit calculated mol wt ~18.5 kDa (LT- beta)
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P01374
• storage temp.: −20°C
• Gene Information: human ... LTA(4049) , LTB(4050)

Description

Biochem/physiol Actions

Lymphotoxin-α (LT-α), also known as tumor necrosis factor-β (TNF-β), and lymphotoxin-β (LT-β) are members of the TNF family. Secreted LT-α complexes with the membrane-associated LT-β to generate two types of heterotrimers, LTα1/β2 and LTα2/β1. The predominant membrane-bound heterotrimer LTα1/β2 binds and activates only to the lymphotoxin β receptor (LT-βR). The other heterotrimer LTα2/β1 binds and activates TNF RI, TNF RII, and LT-βR. LT is critical for normal lymphoid organ development. LT is expressed by activated naive CD4 cells, unpolarized IL-2-secreting effectors, and Th1 effector cells.

Mutations in LTA (lymphotoxin-α) are associated with myasthenia gravis.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured in a cytotoxicity assay using the TNF-susceptible mouse L929 cell line in the presence of actinomycin D.

Safety Information

• Storage Class: 11 - Combustible Solids