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L3289

Sigma-Aldrich

Lysis solution for blood

sufficient for 100 reactions, for molecular biology

Synonym(s):

Blood direct PCR, Blood lysis buffer, Extract-N-Amp blood direct PCR lysis solution

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.25

grade

for molecular biology

form

liquid

usage

sufficient for 100 reactions

storage temp.

−20°C

General description

Lysis solution for blood in combination with neutralization solution for blood (N9784) aids in rapid extraction and neutralization of DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

This lysis solution is a component of the Extract-N-Amp and the REDExtract-N-Amp Blood PCR Kits

Application

Lysis solution for blood has been used for lysis of blood cells for DNA extraction prior to polymerase chain reaction (PCR) amplification. It has also been used for the isolation of genomic DNA for CRISPR-Cas9 gene editing.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Identification by random mutagenesis of functional domains in KREPB5 that differentially affect RNA editing between life cycle stages of Trypanosoma brucei
McDermott S M, et al.
Molecular and Cellular Biology, MCB-00790 (2015)
Identification by random mutagenesis of functional domains in KREPB5 that differentially affect RNA editing between life cycle stages of Trypanosoma brucei
McDermott S M, et al.
Molecular and cellular biology, MCB-00790 (2015)
Shuhan Chen et al.
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Mutations in the Leucine rich repeat kinase 2 (LRRK2) gene are found in both familial and sporadic Parkinson's disease (PD), and are also associated with immune-related disorders including Crohn's disease (CD) and leprosy. We have generated two homozygous LRRK2 knockout
Meng Zhang et al.
Stem cell research, 41, 101602-101602 (2019-11-08)
Loss of function mutations in PARK2 (encoding PARKIN) cause autosomal recessive Parkinson's disease (PD), which often manifests at a juvenile age. Molecular and biochemical studies show that PARKIN functions as an E3 ubiquitin ligase controlling mitochondrial homeostasis. Yet, the exact
Huiling Wu et al.
The Journal of clinical investigation, 117(10), 2847-2859 (2007-09-15)
Ischemia/reperfusion injury (IRI) may activate innate immunity through the engagement of TLRs by endogenous ligands. TLR4 expressed within the kidney is a potential mediator of innate activation and inflammation. Using a mouse model of kidney IRI, we demonstrated a significant

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