L2920
Anti-LPP antibody, Mouse monoclonal
clone LPP4, purified from hybridoma cell culture
Synonym(s):
Anti-LIM domain-containing preferred translocation partner in lipoma, Anti-Lipoma-preferred partner
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
LPP4, monoclonal
form
buffered aqueous solution
mol wt
antigen ~80 kDa
species reactivity
human
packaging
antibody small pack of 25 μL
concentration
~1.0 mg/mL
technique(s)
western blot: 0.5-1.0 μg/mL using whole extract of human HeLa cells
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... LPP(4026)
General description
Monoclonal Anti-LPP (mouse IgG2a isotype) is derived from the hybridoma LPP4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Lipoma-preferred partner (LPP) gene encodes for the LPP protein, prominently expressed in smooth muscle. This protein contains a proline-rich N-terminal region and three C-terminally located LIN-11, Isl-1, and MEC-3 (LIM) domains. LPP protein is a member of the zyxin family. LPP is localized at sites of cell adhesion such as focal adhesions and cell-cell contacts. The LPP gene is mapped on the human chromosome at 3q27.3-q28.
Specificity
Monoclonal Anti-LPP recognizes human LPP.
Application
Anti-LPP antibody, Mouse monoclonal may be used in immunoblotting.
Biochem/physiol Actions
Lipoma-preferred partner (LPP) protein shuttles between cell adhesion sites and the nucleus where it has transcriptional activation capacity. LPP interacts with α-actin and VASP at cell adhesions via its N-terminal. It also interacts with tumor suppressor protein scrib at cell-cell contacts. The LPP gene is being rearranged in a subgroup of lipomas, which are benign tumors of adipose tissue. The LPP gene acts as a translocation partner in acute monoblastic leukemia.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Marleen M R Petit et al.
BMC cell biology, 6(1), 1-1 (2005-01-15)
At sites of cell adhesion, proteins exist that not only perform structural tasks but also have a signaling function. Previously, we found that the Lipoma Preferred Partner (LPP) protein is localized at sites of cell adhesion such as focal adhesions
W Huang et al.
Balkan journal of medical genetics : BJMG, 22(1), 5-10 (2019-09-17)
Copy number variation (CNV) is a main cause of male infertility, yet its influence still remains elusive in that of females. To investigate the correlation between CNV and female infertility, we applied whole-genome CNV analyses by next generation Sequencing (NGS)
Marleen M R Petit et al.
The Journal of biological chemistry, 278(4), 2157-2168 (2002-11-21)
Targeting of proteins to a particular cellular compartment is a critical determinant for proper functioning. LPP (LIM-containing lipoma-preferred partner) is a LIM domain protein that is localized at sites of cell adhesion and transiently in the nucleus. In various benign
M M Petit et al.
Molecular biology of the cell, 11(1), 117-129 (2000-01-19)
The LPP gene is the preferred translocation partner of the HMGIC gene in a subclass of human benign mesenchymal tumors known as lipomas. Here we have characterized the LPP gene product that shares 41% of sequence identity with the focal
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