L2886
Lectin from Lycopersicon esculentum (tomato)
lyophilized powder
Synonym(s):
Lycopersicon esculentum agglutinin, LEA
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About This Item
UNSPSC Code:
12352200
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form
lyophilized powder
potency
<2 μg per mL agglutination activity
composition
Lectin, ≥40%
storage temp.
2-8°C
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Biochem/physiol Actions
LEA is not blood group specific, but has an affinity for N-acetyl-β-D-glucosamine oligomers. The lectin is a glycoprotein containing approx. equal amounts of protein and carbohydrate and is reported to inhibit the mitogenic activity of phytohemagglutinin from Phaseolus vulgaris. This lectin binds endothelial cells and is used to visualize the microvasculature.
Physical form
Contains NaCl
Preparation Note
Purified by affinity chromatography.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.3, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25 °C.
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Di Xie et al.
Glia, 64(4), 583-602 (2015-12-19)
Neuroinflammation elicited by microglia plays a key role in periventricular white matter (PWM) damage (PWMD) induced by infectious exposure. This study aimed to determine if microglia-derived interleukin-1β (IL-1β) would induce hypomyelination through suppression of maturation of oligodendrocyte progenitor cells (OPCs)
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