L2766
Lectin from Datura stramonium (jimson weed, thorn apple)
lyophilized powder
Synonym(s):
Datura stramonium lectin, DSL
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About This Item
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form
lyophilized powder
Quality Level
potency
≤10 μg per mL agglutination activity
composition
Protein, ≥60% biuret
impurities
salt, essentially free
storage temp.
−20°C
General description
Lectin is present in several plants and some animals. It is a tetramer composed of identical sub-units.[1]
Application
Biochem/physiol Actions
DSL is not blood group specific but has an affinity for oligomers of N-acetyl-glucosamine and N-acetyllactosamine. The lectin is a glycoprotein containing approx. 35% carbohydrate.
Lectin identifies and binds to carbohydrate complexes that extend from glycolipids and glycoproteins. It also binds to free-floating glycans, such as monosaccharides.[4]
Quality
Highly purified.
Preparation Note
Purified by affinity chromatography
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution in phosphate buffered saline, pH 6.8. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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