Ricinus communis agglutinin (RCA) occurs in two forms designated RCA60 and RCA120 according to their mol. wt. of approx. 60,000 and 120,000 respectively.1 Neither RCA60 nor RCA120 is blood group specific. RCA60, also referred to as RCAII,2 Ricin D3 or RCL III4 is extremely toxic, inhibits protein synthesis and has an affinity for N-acetyl-D-galactosamine. RCA120, also referred to as RCAI2 or RCL (I + II)4 is an agglutinin and has an affinity for terminal β-D-galactosyl residues.1 Conjugates are prepared from affinity purified toxin, RCA60 or agglutinin RCA120.
Packaging
Package size based on protein content
Unit Definition
One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20°C.
Physical form
Lyophilized powder containing sodium citrate
Preparation Note
Prepared from peroxidase type VI using two step glutaraldehyde method of Avrameas, S. and Ternyck, T., Immunochemistry, 8, 1175 (1971). Repurified after conjugation by affinity chromatography.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2, of a 1 mg protein per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25°C.
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