L1795

Anti-PLAGL1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-LOT-1, Anti-Lost on transformation 1, Anti-PLAL1, Anti-Pleiomorphic adenoma-like protein 1, Anti-Tumor supressor ZAC
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~50 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 0.5-1 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human PLAGL1; western blot: 1-2 μg/mL using lysates of HEK-293T cells over expressing human PLAGL1
• UniProt accession no.: Q9UM63
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PLAGL1(5325)

Description

General description

The gene PLAGL1 (pleiomorphic adenoma-like protein 1), PLAGL2 and PLAG1 form a subfamily of PLAG family of zinc finger proteins that recognize DNA and RNA. The three members share similarity at the amino-terminal, but are functionally different, varying in their DNA binding capacities. The gene is mapped to human chromosome 6q24.2, a region implicated in several cancers.

Specificity

Anti-PLAGL1 (C-terminal) specifically recognizes human PLAGL1.

Application

Anti-PLAGL1 (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

PLAGL1 gene expression is downregulated in a methylation-sensitive manner. PLAGL1 is an imprinted gene that mediates an imprinted gene network that plays an important role in embryonic growth control.

The maternally-imprinted gene, PLAGL1 (pleiomorphic adenoma-like protein 1), functions as an oncogene as well as a tumor suppressor depending on the cell context. It has been found to exhibit anti-proliferative properties and regulate apoptosis and cell-cycle arrest via p53.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.