L0638
Lactate Oxidase from Pediococcus sp.
lyophilized powder, ≥20 units/mg solid
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About This Item
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form
lyophilized powder
specific activity
≥20 units/mg solid
storage temp.
−20°C
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General description
Formerly E.C. number 1.1.3.2
Application
Lactate Oxidase from Pediococcus sp. has been:
- used to functionalize the detection reservoirs for lactate analysis[5]
- used in sequential enzymatic reactions to measure lactate production in the supernatants of microglial cultures[6]
- immobilized onto a carbon electrode to generate a self-powered lactate sensor[4]
Biochem/physiol Actions
Lactate oxidase is used in immobilization or enzyme-based labels for designing biochemical analytical devices such as immunosensors.[5][7] This enzyme catalyzes the oxidation of lactate to produce pyruvate and H2O2.[7] Lactate oxidase is useful in the first-generation biosensor approach for the electrochemical detection of lactate.[4]
Unit Definition
One unit will oxidize 1.0 μmole of L-lactate to pyruvate and H2O2 per min at pH 6.5 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Marcelo Ricardo Romero et al.
Analytical chemistry, 82(13), 5568-5572 (2010-06-04)
An amperometric sensor for lactate quantification is presented. The developed biosensor requires only 0.2 U of lactate oxidase, which is immobilized in a mucin/albumin hydrogel matrix. By protecting the platinum surface with a Nafion membrane, typical interference related to negatively
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By making use of the aggregation-induced emission feature of silole 1 and the cascade reactions among l-lactic acid (LA), lactate oxidase (LOD), and dodecanoic hydrazine (DH), a new fluorometric "turn-on" method is developed for the detection of LA.
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In this work, a novel simple and sensitive electrochemical immunosensor was developed based on lactate oxidase as a single electrochemical probe for the detection of carcinoma antigen 125 (CA125). Chitosan-gold nanoparticle/multiwall carbon nanotube/graphene oxide (CS-AuNP/MWCNT/GO) was used as the electrode
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Biochemistry and biophysics reports, 5, 35-54 (2015-11-11)
Lactate detection plays a significant role in healthcare, food industries and is specially necessitated in conditions like hemorrhage, respiratory failure, hepatic disease, sepsis and tissue hypoxia. Conventional methods for lactate determination are not accurate and fast so this accelerated the
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