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L0377

Sigma-Aldrich

L-Lactic Dehydrogenase from bovine heart

Type IX, Predominantly the LDH H4 isotype, ammonium sulfate suspension, ≥300 units/mg protein

Synonym(s):

(S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD

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About This Item

CAS Number:
9001-60-9
Enzyme Commission number:
1.1.1.27 (BRENDA, IUBMB)
EC Number:
232-617-8
MDL number:
MFCD00131466
UNSPSC Code:
12352204

type

Type IX

form

ammonium sulfate suspension

specific activity

≥300 units/mg protein

UniProt accession no.

P19858
Q5E9B1

foreign activity

glutamic-pyruvic transaminase <0.02%
pyruvate kinase, myokinase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase <0.01%

storage temp.

2-8°C

Gene Information

cow ... LDHA(281274) , LDHB(281275)

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Biochem/physiol Actions

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Unit Definition

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Physical form

Crystalline suspension in 2.1 M (NH4)2SO4 solution, pH 6.0

Analysis Note

Protein determined by biuret

comparable product

Product No.
Description
Pricing

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
057K7500
057K7435
129H7440
129F9630
063H9570

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D Steffens et al.
Journal of biomedical nanotechnology, 9(4), 710-718 (2013-04-30)
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Biochemical pharmacology, 85(11), 1644-1654 (2013-04-16)
NADPH oxidase Nox4 is expressed in a wide range of tissues and plays a role in cellular signaling by providing reactive oxygen species (ROS) as intracellular messengers. Nox4 oxidase activity is thought to be constitutive and regulated at the transcriptional
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Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan, 33(1), 85-91 (2013-04-20)
To determine the cardioprotective effect of magnesium lithospermate B (MLB) on myocardial ischemia/reperfusion (MI/R) injury and to investigate the antioxidant potential in vivo and in vitro. MI/R injury was induced by the occlusion of left anterior descending coronary artery for
O V Basharina et al.
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