K4263
Anti-Kaiso antibody ,Mouse monoclonal
clone 6F, purified from hybridoma cell culture
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About This Item
MDL number:
UNSPSC Code:
12352203
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
6F, monoclonal
form
buffered aqueous solution
species reactivity
human, mouse, rat, canine, chicken
technique(s)
microarray: suitable
western blot: 1-2 μg/mL using CSMLO (mouse mammary adenocarcinoma) cell extracts
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ZBTB33(10009)
mouse ... Zbtb33(56805)
rat ... Zbtb33(501506)
General description
Monoclonal Anti-Kaiso (mouse IgG1 isotype) is derived from the 6F hybridoma produced by the fusion of mouse myeloma cells and splenocytes from A/J mice. Kaiso is a DNA-binding protein and a member of the broad complex, tramtrak, bric a brac/ pox zinc finger (BTB/POZ) family of transcription factors with zinc finger motifs. This protein contains three carboxy-terminal zinc-finger motifs of the Kruppel-like C2H2-DNA binding type zinc fingers and a poxvirus and zinc finger (POZ) domain in its extreme N-terminus, which is highly hydrophobic. The zinc finger and BTB domain containing 33 (ZBTB33) gene is located on the human chromosome at Xq24.
Specificity
Monoclonal Anti-Kaiso reacts specifically with human, rat, mouse, canine, and chicken Kaiso (approx.105 kDa).
Immunogen
recombinant purified mouse Kaiso protein (amino acids 1-499).
Application
Anti-Kaiso antibody, Mouse monoclonal may be used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunocytochemistry
Biochem/physiol Actions
Kaiso is a transcriptional repressor that recognizes specifically the DNA sequence TCCTGCNA or two symmetrically methylated CpG dinucleotides. This protein binds to the cell adhesion cofactor p120 ctn which signifies its role in the p120 ctn action and signaling.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
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Filip Majer et al.
American journal of medical genetics. Part A, 182(1), 219-223 (2019-11-16)
Cullin 4B (CUL4B), lysosomal-associated membrane protein Type 2 (LAMP2), ATP1B4, TMEM255A, and ZBTB33 are neighboring genes on Xq24. Mutations in CUL4B result in Cabezas syndrome (CS). Male CS patients present with dysmorphic, neuropsychiatric, genitourinary, and endocrine abnormalities. Heterozygous CS females
Juliet M Daniel et al.
Nucleic acids research, 30(13), 2911-2919 (2002-06-28)
The p120(ctn)-binding partner Kaiso is a new member of the POZ-zinc finger family of transcription factors implicated in development and cancer. To understand the role of Kaiso in gene regulation and p120(ctn)-mediated signaling and adhesion, we sought to identify Kaiso-specific
The catenin p120(ctn) interacts with Kaiso, a novel BTB/POZ domain zinc finger transcription factor.
J M Daniel et al.
Molecular and cellular biology, 19(5), 3614-3623 (1999-04-17)
p120(ctn) is an Armadillo repeat domain protein with structural similarity to the cell adhesion cofactors beta-catenin and plakoglobin. All three proteins interact directly with the cytoplasmic domain of the transmembrane cell adhesion molecule E-cadherin; beta-catenin and plakoglobin bind a carboxy-terminal
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