K4261
Monoclonal Anti-KSR (Kinase Suppressor of Ras) antibody produced in rat
~2 mg/mL, clone C3H7D2, purified immunoglobulin, buffered aqueous solution
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About This Item
MDL number:
UNSPSC Code:
12352203
Recommended Products
biological source
rat
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
C3H7D2, monoclonal
form
buffered aqueous solution
mol wt
antigen ~100 kDa
species reactivity
mouse
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable using methanol-acetone fixation
microarray: suitable
western blot: 0.5-1 μg/mL using a whole extract of transfected 293T (human embryonal kidney) cells expressing mouse KSR
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Ksr1(16706)
General description
Kinase Suppressor of Ras (KSR) is a positive effector of Ras signalling pathway. Initially discovered in Drosophila and C. elegans, the KSR family of proteins is structurally similar but distinct from the Raf Family. In response to Ras activation, KSR translocates from the cytoplasm to the plasma membrane and may act as a scaffolding protein to assemble a MAPK signalling complex. KSR interacts with Raf-1, MEK1/2 and ERK1/2 and this interaction is required for a signalling cascade downstream of Ras. Thus KSR plays an important role in survival, proliferation, differentiation, growth and transformation of cells. KSR mediates Ras-dependent signals that are required for neuronal differentiation and functioning of central nervous system
Monoclonal Anti-KSR specifically reacts with mouse KSR (~100 kDa).
Monoclonal Anti-KSR specifically reacts with mouse KSR (~100 kDa).
Immunogen
recombinant KSR fragment (amino acids 118-248) from mouse.
Application
Anti-KSR is suitable for immunoblotting at a working concentration of 0.5-1.0 μg/mL using human 293T cells expressing mouse KSR. The antibody may be used for immunocytochemistry and protein microarray applications.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Purified from culture supernatant of hybridoma cells grown in a bioreactor.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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K Kornfeld et al.
Cell, 83(6), 903-913 (1995-12-15)
By screening for mutations that suppress the vulval defects caused by a constitutively active let-60 ras gene, we identified six loss-of-function alleles of ksr-1, a novel C. elegans gene. Our genetic analysis showed ksr-1 positively mediates Ras signaling and functions
N R Michaud et al.
Proceedings of the National Academy of Sciences of the United States of America, 94(24), 12792-12796 (1997-12-16)
Kinase suppressor of Ras (KSR) is an evolutionarily conserved component of Ras-dependent signaling pathways. Here, we find that murine KSR (mKSR1) translocates from the cytoplasm to the plasma membrane in the presence of activated Ras. At the membrane, mKSR1 modulates
D K Morrison
Journal of cell science, 114(Pt 9), 1609-1612 (2001-04-20)
Kinase Suppressor of Ras (KSR) is an intriguing component of the Ras pathway that was first identified by genetic studies performed in Drosophila melanogaster and Caenorhabditis elegans. In both organisms, inactivating mutations in KSR suppress the phenotypic effects induced by
J Müller et al.
Molecular and cellular biology, 20(15), 5529-5539 (2000-07-13)
Kinase suppressor of Ras (KSR) is an evolutionarily conserved component of Ras-dependent signaling pathways. Here, we report the identification of B-KSR1, a novel splice variant of murine KSR1 that is highly expressed in brain-derived tissues. B-KSR1 protein is detectable in
S Stewart et al.
Molecular and cellular biology, 19(8), 5523-5534 (1999-07-20)
Genetic screens for modifiers of activated Ras phenotypes have identified a novel protein, kinase suppressor of Ras (KSR), which shares significant sequence homology with Raf family protein kinases. Studies using Drosophila melanogaster and Caenorhabditis elegans predict that KSR positively regulates
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