J3020

Anti-JAB1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-Jun activation domain-binding protein-1
• MDL number: MFCD03455336
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 38 kDa
• species reactivity: human, mouse, rat
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using sections of human breast carcinoma; immunoprecipitation (IP): 4-6 μg using RIPA extract (0.5 mg) of cultured rat pheochromocytoma PC12 cells; microarray: suitable; western blot: 0.25-0.5 μg/mL using whole extract of cultured mouse NIH3T3 fibroblasts
• UniProt accession no.: Q92905
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... COPS5(10987) ; mouse ... Cops5(26754) ; rat ... Cops5(312916)

Description

General description

JAB1 is highly conserved in evolution and is widely expressed in mammalian tissues. This protein is present in both the nucleus and the cytoplasm.

The gene JAB1 (Jun activation domain-binding protein-1) encodes a component of COP9 signalosome that functions in the phosphorylation of specific transcription factors. The gene is mapped to human chromosome 8q13.2. The encoded protein contains 334 amino acids with a molar mass of 38kDa.

Specificity

Anti- JAB1 recognizes human, mouse and rat JAB1.

Immunogen

synthetic peptide corresponding to amino acid residues 4-20 of human JAB1 with C-terminal added cysteine conjugated to KLH. The sequence is identical in mouse and rat.

Application

Anti-JAB1 antibody produced in rabbit has been used in:

• western blotting
• immunofluorescence
• immunoprecipitation
• immunohistology

Biochem/physiol Actions

The gene JAB1 (Jun activation domain-binding protein-1), also referred to as COPS5 (COP9 subunit 5), is a coactivator of AP-1 (activator protein 1) transcription factor. It is also involved in the regulation of cell cycle by degradation of the cyclin-dependent kinase inhibitor p27Kip1. It increases the levels of endogenous phospho-c-Jun by activating c-Jun amino-terminal kinase (JNK) activity. These activities are inhibited by association with macrophage migration inhibitory factor (MIF), a critical cytokine in several inflammatory responses. This binding, however, does not affect induction of the transcription factor NF (nuclear factor)-κB. It exists as a monomer and functions in deneddylation of NEDD8 (neural precursor cell expressed, developmentally down-regulated 8). It facilitates the shuttling of certain molecules between the nucleus and cytoplasm. JAB1 regulates cellular proliferation and apoptosis by modulating intracellular signaling. Overexpression of this protein has been associated with tumorigenic process.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Storage and Stability

For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)