IP0020
Anti-c-Myc Immunoprecipitation Kit
sufficient for 50 assays
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.56
antibody product type
primary antibodies
usage
sufficient for 50 assays
analyte chemical class(es)
proteins (c-Myc)
technique(s)
immunoprecipitation (IP): suitable
storage temp.
2-8°C
General description
Immunoprecipitation is a method by which a protein can be specifically purified from a complex mixture of proteins using a specific antibody and a matrix that binds the antibody. The matrix bound protein can then be separated from the mixture by centrifugation. IP followed by SDS-PAGE and immunoblotting are routinely used in a variety of applications to measure the molecular masses of protein antigens, study protein interactions, determine specific enzymatic activity, monitor protein post-translation modifications, and determine the presence and quantity of proteins. The IP technique also enables the detection of rare proteins, which otherwise would be difficult to detect, since immunoprecipitation can concentrate them to as much as 10,000-fold.
Application
Specially designed to allow maximal recovery of c-Myc-tagged proteins in the immunoprecipitates. The whole process is performed in mini-spin columns, instead of in microcentrifuge tubes, thus combining convenient washing of the antigen-antibody-bound beads with minimal variability between experiments.
Anti-c-Myc immunoprecipitation kit was used in the identification of epithelial stromal interaction 1 as a novel effector downstream of Krüppel-like factor 8 in breast cancer invasion and metastasis.[1] It was also used to study the CD81 interaction with the T cell receptor to suppress signaling.[2]
Anti-c-Myc immunoprecipitation kit was used in the identification of epithelial stromal interaction 1 as a novel effector downstream of Krüppel-like factor 8 in breast cancer invasion and metastasis.[1] It was also used to study the CD81 interaction with the T cell receptor to suppress signaling.[2]
Features and Benefits
- Spin columns ensures more consistent results
- Anti-c-Myc Affinity Gel ensures high specific binding to c-Myc-tagged fusion protein
- CelLytic™ M Cell Lysis Reagent for rapid and efficient mammalian cell lysis
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
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Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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