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I9285

Sigma-Aldrich

IsoQuick™ Strips for Mouse Monoclonal Isotyping

IgG1, IgG2a, IgG2b, IgG3

Synonym(s):

Mouse Monoclonal Antibody Isotyping Reagents, IgG Isotyping for Mouse Monoclonals

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About This Item

MDL number:
MFCD08705607
UNSPSC Code:
12352203

storage temp.

2-8°C

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Specificity

Mouse IgG1, IgG2a, IgG2b, and IgG3 isotype subclasses.

Application

Determination of the subclass of a monoclonal antibody is helpful for characterization of the antibody, for choosing detection reagents, and for deciding on a purification scheme.
The IsoQuick strips, whether purchased in kits (ISOQ5 and ISOQ25) or separately (I9285, I9410, or I9535), are designed to detect and identify mouse isotypes in cell culture supernatant, ascites, or purified antibody. Optimum detection range is 0.1-4 μg/mL with a detection limit of 10 ng/mL. IsoQuick strips have been validated for use with mouse monoclonal antibodies and have not been validated for use with other host antibodies.

Features and Benefits

The strips come ready-to-use and provide results in 5 minutes or less. The IsoQuick strips can be used individually or in combination with the same prepared sample for customized results.

Other Notes

The IsoQuick IgG strips are designed to detect and identify mouse IgG1, IgG2a, IgG2b, and IgG3 isotype subclasses.

Legal Information

IsoQuick is a trademark of EnviroLogix Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLBD1875V
07M0918
069K0784
069K0785

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can I use IsoQuick™  strips to classify the antibody light chains?

    Yes, Product No. I9535 is for distinguishing kappa and lambda light chains.  These strips can be ordered separately.  They are provided in the ISOQ5 and ISOQ25 kits.

  6. With what types of samples will IsoQuick™  strips work?

    The IsoQuick strips can be used in cell culture supernatant with no dilution or other preparation. If testing ascites the sample should be diluted in a range from 1:10,000 to 1:100,000. If testing purified antibody, the sample should be diluted to a concentration of 0.1 to 4.0 μg/mL.

  7. How sensitive is IsoQuick™ ?

    The sensitivity is quite good; each IsoQuick strip can detect as little as 0.1 μg/mL

  8. Can IsoQuick™ strips be reused?

    No. The strips can only be used once, even if all the test lines did not develop.

  9. Are the IsoQuick™ strips light sensitive?

    Yes. The strips should be kept in the desiccant tube they were delivered in to protect them from light contamination. Prolonged exposure to UV or other light sources may affect the visibility of the control and test lines on the device.

  10. Are the IsoQuick™ strips humidity sensitive?

    Yes. The strips should be kept in the desiccant tube they were delivered in to ensure the proper humidity level. Strips left outside the tube in a high humidity environment may result in test errors.

  11. Are the IsoQuick™ strips heat sensitive?

    Yes. The strips should be kept in the desiccant tube and stored at 2-8°C. This will ensure maximum useful life. IsoQuick strips can be stored at room temperature, but this will diminish their useful life. If the strips are exposed to extreme hot or cold environments the performance of the strip is likely to be effected.

  12. When using IsoQuick™ strips, do I need to dilute my samples?

    No dilution is required when using the IsoQuick in cell culture supernatant. Place strips directly into the sample. To use IsoQuick strips in ascites dilute the sample to a concentration of 1:10,000 to 1:100,000. To use IsoQuick strips to test purified antibody dilute the sample to a concentration of  0.1 - 4.0 μg/mL.

  13. Can I use the IIsoQuick™ strips to determine the antibody titer?

    IsoQuick IgG strips can be used for semi-quantitative determination of the IgG titer in a cell supernatant sample. For semi-quantitative measurement, run IsoQuick strips with standards of known titer (1,000 μg/mL, 100 μg/mL, and 10 μg/mL). Then compare the color of the result line for the sample to those obtained with the standards (visually or using a color absorbance reader). Color intensity is representative of sample titer.

  14. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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