IKK α is a component of I-κ-B-kinase (IKK) core complex consisting of IKK α, IKK β and IKK γ and belongs to Ser/Thr protein kinase family. It has a pivotal role in NF-κ-B signaling pathway. Anti-IKK α antibody can be used in immunoblot for detecting IKKα (α subunit of IκB kinase). Rabbit anti-IKK α antibody reacts specifically with IKK α (85kD) and has shown no cross reactivity with IKK β or IKK γ.
Immunogen
synthetic peptide corresponding to amino acids 716-734 of human IκB Kinase α (IKKα) (C1).
Application
Anti-IKK α antibody can be used in western blotting (diluted 1:1000) using human HeLa cell extract.
Physical form
Solution in phosphate buffered saline containing 0.02% sodium azide.
Nuclear transcription factors of the NF-kappaB/Rel family are inhibited by IkappaB proteins, which inactivate NF-kappaB by trapping it in the cell cytoplasm. Phosphorylation of IkappaBs marks them out for destruction, thereby relieving their inhibitory effect on NF-kappaB. A cytokine-activated protein
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