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Key Documents

I7903

Sigma-Aldrich

Anti-IKKα (716-734) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-IκB Kinase α

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 85 kDa

species reactivity

human

technique(s)

western blot: 0.5 μg/mL using human HeLa cell extract

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CHUK(1147)

General description

IKK α is a component of I-κ-B-kinase (IKK) core complex consisting of IKK α, IKK β and IKK γ and belongs to Ser/Thr protein kinase family. It has a pivotal role in NF-κ-B signaling pathway. Anti-IKK α antibody can be used in immunoblot for detecting IKKα (α subunit of IκB kinase). Rabbit anti-IKK α antibody reacts specifically with IKK α (85kD) and has shown no cross reactivity with IKK β or IKK γ.

Immunogen

synthetic peptide corresponding to amino acids 716-734 of human IκB Kinase α (IKKα) (C1).

Application

Anti-IKK α antibody can be used in western blotting (diluted 1:1000) using human HeLa cell extract.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

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Storage Class

10 - Combustible liquids


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J A DiDonato et al.
Nature, 388(6642), 548-554 (1997-08-07)
Nuclear transcription factors of the NF-kappaB/Rel family are inhibited by IkappaB proteins, which inactivate NF-kappaB by trapping it in the cell cytoplasm. Phosphorylation of IkappaBs marks them out for destruction, thereby relieving their inhibitory effect on NF-kappaB. A cytokine-activated protein

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