I7778
Anti-IKKα (699-715) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-IκB Kinase α
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About This Item
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 85 kDa
species reactivity
human
technique(s)
western blot: 1 μg/mL using human HeLa cell extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CHUK(1147)
mouse ... Chuk(12675)
General description
The NF-κB transcription factors are presented inactive in the cytoplasm by inhibitor protein IκB. In response to stimuli such as UV light or inflammatory cytokines, phosphorylation and subsequent degradation of IκB, allows for nuclear translocation of NF-κ B where it functions as transcription factor. IκB is phosphorylated by a serine kinase, IκB kinase (IKK). The IKK consists of three subunits, IKKα and IKKβ are the catalytic, IKKγ is a regulatory subunit. IKKα and its upstream kinase, NIK are the key kinases in the activation of the alternate or the non-canonical NFκB pathway. IKKα has been reported to function independent of either IKKβ or IKKγ in activation of NF-κB. IKKα also shows functions other than activation of NF-κ B. It binds the promoters of estrogen-responsive genes, Cyclin D1 and c-myc
Anti-IKKα?detects IKKα?(85 kDa) and has no cross-reactivity with IKKβ?or IKKγ.
Anti-IKKα?detects IKKα?(85 kDa) and has no cross-reactivity with IKKβ?or IKKγ.
Immunogen
synthetic peptide corresponding to amino acids 699-715 of human IκB Kinase α (IKK α) (C2). This sequence contains one amino acid substitution with the mouse sequence.
Application
Anti-IKK α subunit may be used for detection by immunoblotting at a recommended concentration of 1 μg/mL or 1:500 dilution using HeLa whole cell lysates.
Physical form
Solution in phosphate buffered saline containing 0.02% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
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C H Régnier et al.
Cell, 90(2), 373-383 (1997-07-25)
Activation of the transcription factor NF-kappaB by tumor necrosis factor (TNF) and interleukin-1 (IL-1) requires the NF-kappaB-inducing kinase (NIK). In a yeast two-hybrid screen for NIK-interacting proteins, we have identified a protein kinase previously known as CHUK. Overexpression of CHUK
Shao-Cong Sun
Cell research, 21(1), 71-85 (2010-12-22)
The non-canonical NF-κB pathway is an important arm of NF-κB signaling that predominantly targets activation of the p52/RelB NF-κB complex. This pathway depends on the inducible processing of p100, a molecule functioning as both the precursor of p52 and a
Alain Israël
Cold Spring Harbor perspectives in biology, 2(3), a000158-a000158 (2010-03-20)
The IKK kinase complex is the core element of the NF-kappaB cascade. It is essentially made of two kinases (IKKalpha and IKKbeta) and a regulatory subunit, NEMO/IKKgamma. Additional components may exist, transiently or permanently, but their characterization is still unsure.
Yee Mon Thu et al.
Cytokine & growth factor reviews, 21(4), 213-226 (2010-08-06)
NF-κB inducing kinase (NIK) is a kinase that activates the canonical and non-canonical NF-κB pathways to control transcriptional expression of certain proteins such as cytokines, chemokines and NF-κB signaling molecules. Many advances have been made in understanding the molecular mechanisms
U Senftleben et al.
Science (New York, N.Y.), 293(5534), 1495-1499 (2001-08-25)
In mammals, the canonical nuclear factor kappaB (NF-kappaB) signaling pathway activated in response to infections is based on degradation of IkappaB inhibitors. This pathway depends on the IkappaB kinase (IKK), which contains two catalytic subunits, IKKalpha and IKKbeta. IKKbeta is
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