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I7267

Sigma-Aldrich

Insect Medium Supplement (10×)

liquid, sterile-filtered, BioReagent, suitable for insect cell culture

Synonym(s):

10× Insect Supplement, Insect Medium, Medium Supplement

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | insect: suitable

storage temp.

2-8°C

Application

A serum replacement for general cell growth and maintenance.
May be used in the preparation of low protein medium. In many instances the level of serum can be reduced by 50-75% in cultures containing 10% medium supplement. The low protein content (<150 μg/ml) also makes the product suitable for use in preparing low protein serum-free medium. Serum-free medium can be prepared by adding the medium supplement to a final concentration of 10% to an appropriate cell culture medium. The final protein content of the medium will be dependent upon the level of proteins (if any) present in the basal medium. In many instances the adaptation of cells to growth in serum-free medium may be required. Also recommended for use with serum to improve cell growth and maintenance.

Other Notes

Proprietary mixture of amino acids, lipids, trace elements, vitamins and other growth enhancing components in an aqueous solution.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Jeong-Hoon Han et al.
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Emily Tubman et al.
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The microtubule motor protein kinesin-5 is well known to establish the bipolar spindle by outward sliding of antiparallel interpolar microtubules. In yeast, kinesin-5 also facilitates chromosome alignment "congression" at the spindle equator by preferentially depolymerizing long kinetochore microtubules (kMTs). The

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