I5020
Anti-Interleukin 10 antibody produced in goat
IgG fraction of antiserum, lyophilized powder
Synonym(s):
Anti-IL-10
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About This Item
Recommended Products
biological source
goat
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
human
technique(s)
neutralization: suitable
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... IL10(3586)
General description
Interleukin 10 is located on human chromosome 1q32.1.
Interleukin-10 (IL-10) is a pleotropic cytokine with anti-inflammatory and immunosuppressive functions. It is the key cytokine that regulates the host inflammatory responses to bacterial, fungal and viral pathogens. The production of IL-10 is mediated by signalling of IL-6, IL-12 and TGF-β. IL-10 is secreted by a variety of cells including T cells, B cells, dendritic cells, eosinophils, natural killer cells and mast cells. The effects of IL-10 are mediated by binding to the IL-10R, consisting of α and β subunits. The signalling through IL-10R activates Jak1, Tyk2 protein kinases and JAK/STAT3 pathways. IL-10 is involved in suppression of innate immunity to kill pathogens and inhibits the release of reactive oxygen and nitrogen species from activated macrophages. Regulation of IL-10 production is important in infections, inflammatory responses in autoimmune encephalomyelitis, endotoxic shock and chronic enterocolitis
Anti-interleukin-10 antibody is specific for human IL-10. The antibody shows ~7% cross reactivity with recombinant mouse IL-10 in ELISA and immunoblotting.
Anti-interleukin-10 antibody is specific for human IL-10. The antibody shows ~7% cross reactivity with recombinant mouse IL-10 in ELISA and immunoblotting.
Specificity
The antibody neutralizes the biological activity of recombinant human IL-10, but not recombinant mouse IL-10.
Immunogen
recombinant human IL-10 expressed in Sf 21 insect cells.
Application
Anti-interleukin 10 antibody produced in goat has been used to measure the anti-allodynic effect of IL-10 in the neuropathic pain rat model.
Anti-interleukin-10 antibody may be used for immunoblotting at a working concentration of 1 μg/ml. For ELISA a working concentration of 0.25 μg/ml is recommended. The antibody is suitable for neutralization reactions. This antibody has also been used for immunohistochemistry of cervicovaginal epithelial cells of rabbit
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
Biochem/physiol Actions
Interleukin 10 (IL10) helps to induce anti-tumor immune response. It controls tumor tolerance in non-small cell lung cancer. IL10 prevents the synthesis of pro-inflammatory cytokines by activating the JAK/STAT (janus kinase/signal transducers and activators of transcription) signalling pathway.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.
Preparation Note
Purified using protein G.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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Genetic associations of the interleukin locus at 1q32. 1 with clinical outcomes of cutaneous melanoma.
Rendleman J, et al.
Journal of medical Genetics, 52(4), 231-239 (2015)
Sadhana M Gupta et al.
Mediators of inflammation, 2008, 534532-534532 (2009-01-07)
Topical microbicides are intended for frequent use by women in reproductive age. Hence, it is essential to evaluate their impact on mucosal immune function in the vagina. In the present study, we evaluated nisin, a naturally occurring antimicrobial peptide (AMP)
Interleukin-10-regulated tumour tolerance in non-small cell lung cancer.
Vahl JM, et al.
British Journal of Cancer, 117(11), 1644-1644 (2017)
Toll-Like Receptors and cytokines as surrogate biomarkers for evaluating vaginal immune response following microbicide administration
Gupta SM et al
Mediators of Inflammation, doi:10-doi:10 (2008)
Chlamydia trachomatis inhibits the production of pro-inflammatory cytokines in human PBMCs through induction of IL-10.
Du K, et al.
Journal of Medical Microbiology, 67(2), 240-248 (2018)
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