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I4777

Sigma-Aldrich

Interferon-γ from mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

IFN-γ

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

mouse

Quality Level

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE)

form

lyophilized powder

mol wt

protein 15.6 kDa

packaging

pkg of 0.1 mg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

solubility

water: soluble 1.000 mL, clear, colorless

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Ifng(15978)

Application

Interferon-γ from mouse has been used:
  • for in vitro stimulation of the BMDC (bone marrow derived dendritic cell), BMM (bone marrow-derived macrophages) and CD11c+ (CD11 antigen-like family member C) lung macrophages
  • for stimulation of murine macrophages to induce the production of nitrite and nitrate
  • to check CCAAT/enhancer binding protein β (C/EBPβ)-mediated changes in the expression of prostaglandin synthesis enzymes in neuroinflammation

Biochem/physiol Actions

Ifng (interferon-γ) plays an important role in innate and adaptive immune responses, and helps in defense against bacterial infections, dimorphic yeast and some viral infections.

Physical form

Lyophilized from a 0.2 μm filtered buffered solution. Package size based on protein content.

Analysis Note

The biological activity of recombinant mouse IFN-γ is measured by its ability to inhibit the proliferation of murine WEHI-279 cells.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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Sergio Arias-Negrete et al.
Analytical biochemistry, 328(1), 14-21 (2004-04-15)
Preparation of a nitrate reductase lysate of Escherichia coli MC1061 to measure nitrate and nitrite in biologic fluids is described. To obtain the crude bacterial lysate containing nitrate reductase activity, E. coli MC1061 was subjected to 16-20 freeze-thawing cycles, from
S N Vogel et al.
Infection and immunity, 38(2), 681-685 (1982-11-01)
Silica has been used for many years as an agent which selectively alters macrophage functions and, as such, has been used to assess the role of macrophages in the immune response to a variety of microbial and chemically defined agents.
Anne M Sanders et al.
BMC cancer, 10, 121-121 (2010-04-02)
Spontaneous Regression/Complete Resistant (SR/CR) mice are a colony of cancer-resistant mice that can detect and rapidly destroy malignant cells with innate cellular immunity, predominately mediated by granulocytes. Our previous studies suggest that several effector mechanisms, such as perforin, granzymes, or
Marco Straccia et al.
Glia, 61(10), 1607-1619 (2013-07-31)
The eicosanoid prostaglandin E2 (PGE2 ) plays important roles in neuroinflammation and it is produced by the sequential action of the enzymes cyclooxygenase-2 (COX-2) and prostaglandin E synthase (PTGES). The expression of both enzymes and the production of PGE2 are
Kiwon Park et al.
Journal of leukocyte biology, 88(6), 1081-1087 (2010-08-12)
The oxysterol-producing enzyme CH25H plays an important role in regulating lipid metabolism, gene expression, and immune activation. In vitro experiments using a panel of TLR agonists to activate BMDCs and macrophages demonstrated that Ch25h expression is induced rapidly, selectively, and

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