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Key Documents

I4023

Sigma-Aldrich

Interferon from rat kidney cells stimulated with poly (I:C)

lyophilized powder, ≥1 × 105 IRU/mg protein (IRU = International Reference Unit)

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About This Item

MDL number:
UNSPSC Code:
51111800

biological source

rat

description

non-irradiated, sterile filtered

form

lyophilized powder

specific activity

≥1 × 105 IRU/mg protein (IRU = International Reference Unit)

packaging

pkg of 150K U

storage condition

avoid repeated freeze/thaw cycles

UniProt accession no.

storage temp.

2-8°C

Gene Information

rat ... Ifng(25712)

Unit Definition

One unit protects 50% of the indicator cells from viral cytopathology.

Physical form

Lyophilized from 0.4 M glycine HCl, pH 3.5

Analysis Note

Potency: Established in multiple comparative assays with the interferon reference reagents provided by the Antiviral Substances Program, NIAID, NIH, using a modified dye-uptake assay. The protein concentration was established using the Coomassie blue-binding assay with bovine albumin as a standard.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Peter Hoffmann et al.
Regulatory peptides, 168(1-3), 27-31 (2011-03-10)
Interferon γ (IFNγ) has been originally identified by its anti-viral activity and has been demonstrated to act as potent modulator of the immune system with a range of target cells limited largely to immune cell populations. Although IFNγ has been
Hamid Reza Jafarinaveh et al.
Iranian journal of allergy, asthma, and immunology, 13(2), 125-130 (2013-12-18)
Increased levels of proinflammatory cytokines have been recorded after the onset of transient or permanent brain ischemia and are usually associated with exacerbation of ischemic injury. Embolic stroke model is more relevant to the pathophysiological situation in such patients, because
Finter, N.B.,
The Journal of General Virology, 5, 419-419 (1969)
Ge Hu et al.
Immunopharmacology and immunotoxicology, 32(4), 656-662 (2010-03-11)
Although researchers have recently begun to pay more attention to the immunological characteristics of microvascular endothelial cells (MVECs), there are no reports on whether activation of MVECs by interferon-γ (IFN-γ) exerts any influence on the expressions of IFN-α/β. In the
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M M Bradford
Analytical biochemistry, 72, 248-254 (1976-05-07)

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