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I3782

Sigma-Aldrich

Insulin-like Growth Factor Binding Protein-5 from mouse

≥90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

Synonym(s):

IGFBP-5

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About This Item

MDL number:
MFCD04040883
UNSPSC Code:
51111800

biological source

mouse

recombinant

expressed in NSO cells

assay

≥90% (SDS-PAGE)

form

lyophilized powder

potency

0.5-2.5 μg/mL ED50

mol wt

calculated mol wt 28.9 kDa

packaging

pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)

impurities

endotoxin, tested

UniProt accession no.

Q07079

storage temp.

−20°C

Gene Information

mouse ... Igfbp5(16011)

General description

IGFBP5 (insulin-like growth factor binding protein-5) is a secreted protein and can also move to the nucleus. IGFBPs have cysteine-rich amino- and carboxyl-terminal domains.[1]

Application

As a result of glycosylation, the recombinant mouse protein migrates to at least three bands with molecular weights ranging from 28-38 kDa in SDS-PAGE under reducing conditions.

Biochem/physiol Actions

Binds to bone cells and is the predominant insulin-like growth factor binding protein in bone; when binding the extracellular matrix, it modulates IGF activity.
IGFBP-5 binds to bone cells and is the predominant insulin-like growth factor binding protein in bone. When IGFBP-5 binds the extracellular matrix, it modulates IGF activity. Mouse, human, and rat IGFBP-5 share 97% homology.
IGFBPs (insulin-like growth factor binding proteins) control IGF functions by regulating their access to other proteins, including receptors. IGFBP5 is involved in osteogenesis, muscle differentiation and kidney development.[1] It is a crucial protein for the development of fibrosis.[2]

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% TFA containing 1.25 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to inhibit recombinant mouse IGF-II on MCF-7 cells.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
117K1572
017K1489
092K1574

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Hidekata Yasuoka et al.
PloS one, 9(2), e87754-e87754 (2014-02-20)
Systemic sclerosis (SSc) is characterized by excessive fibrosis of the skin and internal organs due to fibroblast proliferation and excessive production of extracellular matrix (ECM). We have shown that insulin-like growth factor binding protein (IGFBP)-5 plays an important role in
Mahta Nili et al.
The Journal of biological chemistry, 287(2), 1510-1519 (2011-11-26)
The six high-affinity insulin-like growth factor-binding proteins (IGFBPs) comprise a conserved family of secreted molecules that modulate IGF actions by regulating their half-life and access to signaling receptors, and also exert biological effects that are independent of IGF binding. IGFBPs

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