I2408
Anti-phospho-Insulin Receptor Substrate-1 (pTyr896) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
Synonym(s):
Anti-phospho-IRS-1 (pTyr896)
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About This Item
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
species reactivity
rat, human, mouse
technique(s)
western blot: 1:1,000 using stimulated CHO-T cell line
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr896)
Gene Information
human ... IRS1(3667)
mouse ... Irs1(16367)
rat ... Irs1(25467)
General description
Insulin receptor substrate-1 (IRS-1) is among the four IRS proteins (IRS-1-IRS-4) that mediate signalling by insulin receptor (IR) and insulin-like growth factor (IGF) receptor. IRS-1 has a pleckstrin homology domain and a phosphotyrosine-binding domain. Phosphorylation of the Tyr1179 residue in the SH-1 negatively regulates insulin. IRS-1 has the abililty to translocate to the nucleus, the mechanism of which is not very clear. Estrogen receptors (ER) α and β have a strong affinity to IRS-1, an important interaction in breast cancers and medulloblastomas. The expression of IRS-1 in prostate cancer is associated with lower tumor cell invasiveness. Along with ER α, IRS-1 also modulates transcriptional activity involved in the development of breast and prostate cancer. The signalling pathway involving IGF-IR-IRS-1 in cells expressing JCV T-antigen has been reported to affect the proliferation, survival and DNA repair in the cells. The phosphorylation of IRS-1 at Tyr896 is required for IRS-1-Grb 2 association
Anti-phospho-IRS-1 (pTyr8969) specifically recognizes human IRS-1 (pTyr896). It may react with mouse and rat (100% homology) IRS-1.
Anti-phospho-IRS-1 (pTyr8969) specifically recognizes human IRS-1 (pTyr896). It may react with mouse and rat (100% homology) IRS-1.
Immunogen
synthetic phosphopeptide derived from the region of insulin receptor substrate-1 that is phosphorylated on tyrosine 896. The sequence is conserved among multiple species including human, mouse, and rat.
Application
Anti-phospho-IRS-1 (pTyr896) antibody may be used for detection by immunoblotting, in insulin-stimulated CHO cell line, at a working dilution of 1:1000.
Physical form
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), 50% glycerol with 1 mg/mL BSA (IgG, protease free), and 0.05% sodium azide.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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A M Valverde et al.
Molecular and cellular biology, 21(7), 2269-2280 (2001-03-22)
We have recently generated immortalized fetal brown adipocyte cell lines from insulin receptor substrate 1 (IRS-1) knockout mice and demonstrated an impairment in insulin-induced lipid synthesis as compared to wild-type cell lines. In this study, we investigated the consequences of
Catia Morelli et al.
Oncogene, 23(45), 7517-7526 (2004-08-20)
Insulin receptor substrate 1 (IRS-1) is a major signaling molecule activated by the insulin and insulin-like growth factor I receptors. Recent data obtained in different cell models suggested that in addition to its conventional role as a cytoplasmic signal transducer
M G Myers et al.
Endocrinology, 132(4), 1421-1430 (1993-04-01)
IRS-1 is a unique cytosolic protein that becomes tyrosine phosphorylated during insulin stimulation of intact cells and immediately associates with the phosphatidylinositol 3'-kinase (PtdIns 3'-kinase). The insulin-like growth factor-I (IGF-I) receptor also mediated the tyrosine phosphorylation of IRS-1 and increased
X J Sun et al.
The Journal of biological chemistry, 267(31), 22662-22672 (1992-11-15)
IRS-1 is a major insulin receptor substrate which may play an important role in insulin signal transmission. The mRNA for IRS-1 in rat cells and tissues is about 9.5 kilobases (kb). Rat liver IRS-1 was stably expressed in Chinese hamster
Joanna Trojanek et al.
Journal of cellular physiology, 206(1), 35-46 (2005-06-21)
JC polyomavirus (JCV), which infects 90% of the human population, is detectable in human tumors. Its early protein, JCV T-antigen, transforms cells in vitro and is tumorigenic in experimental animals. Although T-antigen-mediated transformation involves genetic alterations of the affected cells
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