HPA011069

Anti-MANEA antibody produced in rabbit

Ab2, Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

• Synonym(s): Anti-Endo-α mannosidase, Anti-Endomannosidase, Anti-Glycoprotein endo-α-1,2-mannosidase, Anti-Mandaselin, Anti-hEndo
• UNSPSC Code: 12352203
• Human Protein Atlas Number: HPA011069
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• product line: Prestige Antibodies^® Powered by Atlas Antibodies
• form: buffered aqueous glycerol solution
• species reactivity: human
• enhanced validation: independent; Learn more about Antibody Enhanced Validation
• technique(s): immunohistochemistry: 1:20- 1:50
• immunogen sequence: GVLALSWYPPDVNDENGEPTDNLVPTILDKAHKYNLKVTFHIEPYSNRDDQNMYKNVKYIIDKYGNHPAFYRYKTKTGNALPMFYVYDSYITKPEKWANLLTTSGSRSIRNSPYDGLFIALLVEEKHKYDILQSGFDGIYT
• UniProt accession no.: Q5SRI9
• shipped in: wet ice
• Storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MANEA(79694)

Description

General description

MANEA (mannosidase, endo-α) is a type II transmembrane glycosidic protein. This enzyme is made of 462 amino acids and has a molecular weight of 53.6kDa. Its transmembrane region is made of 19 amino acids and lies in close proximity to the N-terminal. This gene is present on human chromosome 6q16.1, and is expressed in multiple human tissues including brain, kidney and liver.

Immunogen

Glycoprotein endo-α-1,2-mannosidase recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

MANEA (mannosidase, endo-α) catalyzes the cleavage of mono-, di-, and tri-glucosyl-mannose oligosaccharides from high-mannose N-glycans, by breaking the α-1,2-mannosidic bond. Studies also suggest that MANEA is involved in ensuring proper N-glycosylation, and thus provides cells with the ability to recover and mature glycosylated structures. Single nucleotide polymorphisms (SNPs) in this gene are linked to cocaine dependence and cocaine induced paranoia in European American and African American populations. Studies suggest that variations in MANEA gene are associated with anxiety disorder.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST72260

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)