H8791

Transforming Growth Factor-β3 human

TGF-β3, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture

• Synonym(s): TGF-β3
• MDL number: MFCD00213054
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in HEK 293 cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤1.0 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: dimer 25 kDa (non-glycosylated)
• packaging: pkg of 5 μg
• manufacturer/tradename: HumanZyme
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg
• UniProt accession no.: P10600
• storage temp.: −20°C
• Gene Information: human ... TGFB3(7043)

Description

General description

TGF-β3 (transforming growth factor-β3) belongs to the TGF β superfamily. The TGFβ3 gene is mapped to human chromosome 14q24.3

Biochem/physiol Actions

TGF-β3 (transforming growth factor-β3) regulates lymphocyte proliferation, apoptosis, hematopoiesis and embryogenesis to maintain immune homeostasis. TGF-β plays an important role in cell growth, differentiation, and survival. TGF-β3 specifically promotes chondrogenic differentiation.TGF-β3 is a strong growth inhibitor for normal and transformed epithelial, lymphoid, fibroblast, and keratinocyte cells. TGF-2 inhibits antitumor action of NK (natural killer) cells, T-cells, macrophages, monocytes and neutrophils. TGF-β3 is a tumor suppressor in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis. TGF-β isoforms is known to participate in wound healing and tissue fibrosis. TGF-β3 is crucial for tissue restoration and scarless tissue repair. Mutation in the TGFβ3 gene is associated with development of non-syndromic cleft palate only (NS CPO), a rare congenital disease.

TGF-β3 is less prevalent in natural expression than either TGF-β1 or TGF-β2, but it is the most abundant mRNA expressed in chick embryos. It is also expressed in human umbilical cord, in a variety of mesenchymal cells of human and rodent origin, and in several human carcinoma cells.

Physical form

Lyophilized from a 0.2 μm filtered solution of 50 mM NaOAc pH 4.5

Preparation Note

HumanKine TGF-β3 is expressed in human HEK 293 cells as a mature, disulfide linked, non- glycosylated homodimer with a predicted molecular mass of 25 kDa.
This product is lyophilized from a solution of 50mM NaOAc, pH 3.7.

Analysis Note

The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable