H8142
Hybridoma Enhancing Supplement from murine macrophage-like cell line
liquid, sterile-filtered, suitable for hybridoma
Synonym(s):
Conditioned Medium from murine macrophage-like cell line
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About This Item
UNSPSC Code:
12352205
Recommended Products
sterility
sterile-filtered
Quality Level
form
liquid
technique(s)
cell culture | hybridoma: suitable
impurities
Mycoplasma, tested
endotoxin, tested
shipped in
dry ice
storage temp.
−20°C
Application
Use at a concentration of 10% in complete medium containing FBS (10%) or CPRS-3. Optimal conditions must be determined for specific applications.
Other Notes
Conditioned medium from a proprietary murine macrophage-like cell line grown in IMDM with 2% FBS.
Caution
Avoid repeated freezing and thawing. If entire contents are not used, store frozen in working aliquots. Precipitation may occur when stored. This is not detrimental to the product and may be reversed when warmed to 37 °C.
Analysis Note
Cell line viral testing:
Murine Antibody Production (map) testing for Sendai, MHV, PVM, Reo3, GDVII, Ectomelia, MVM, Polyoma, LDMV, M. Ad, EDIM, MCMV, LCM, Hantaan.
BVD virus by fluorescent antibody stain
Murine Antibody Production (map) testing for Sendai, MHV, PVM, Reo3, GDVII, Ectomelia, MVM, Polyoma, LDMV, M. Ad, EDIM, MCMV, LCM, Hantaan.
BVD virus by fluorescent antibody stain
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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