H7285
Hepatitis C virus NS4 antigen−Biotin
~1 mg/mL, ≥95% (SDS-PAGE), recombinant, expressed in E. coli
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About This Item
recombinant
expressed in E. coli
Quality Level
assay
≥95% (SDS-PAGE)
mol wt
19 kDa (plus 114 kDa β-Gal tag)
concentration
~1 mg/mL
UniProt accession no.
storage temp.
−20°C
Gene Information
hepatitis C virus ... HCVgp1(951475)
Biochem/physiol Actions
Positive control for HCV antibodies.
Other Notes
HCV NS4a+b [1658-1863]-Galactosidase-tagged, biotin conjugate.
Physical form
Solution in 8 M Urea, 20 mM Tris-HCl, pH 8.0, 10 mM 2-Mercaptoethanol.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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R Bartenschlager et al.
Journal of virology, 69(12), 7519-7528 (1995-12-01)
Processing of the hepatitis C virus polyprotein is mediated by host cell signalases and at least two virally encoded proteinases. Of these, the serine-type proteinase encompassing the amino-terminal one-third of NS3 is responsible for cleavage at the four sites carboxy
Miriam T Brady et al.
European journal of immunology, 33(12), 3448-3457 (2003-11-25)
The majority of hepatitis C virus (HCV) infections become chronic, despite the presence of HCV-specific cellular and humoral immune responses. We have previously suggested that IL-10-secreting antigen-specific regulatory T cells may contribute to viral persistence, and demonstrate here that peripheral
Inés Romero-Brey et al.
mBio, 6(4), e00759-e00759 (2015-07-15)
Induction of membrane rearrangements in the cytoplasm of infected cells is a hallmark of positive-strand RNA viruses. These altered membranes serve as scaffolds for the assembly of viral replication factories (RFs). We have recently shown that hepatitis C virus (HCV)
Margaret A Scull et al.
PLoS pathogens, 11(11), e1005297-e1005297 (2015-11-21)
The hepatitis C virus (HCV) p7 protein is required for infectious virus production via its role in assembly and ion channel activity. Although NMR structures of p7 have been reported, the location of secondary structural elements and orientation of the
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