H6537

Monoclonal Anti-Histone Deacetylase 3 (HDAC3) antibody produced in mouse

~1 mg/mL, clone HDAC3-83, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-HD3
• MDL number: MFCD02096545
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: HDAC3-83, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~50 kDa
• species reactivity: mouse, human
• concentration: ~1 mg/mL
• technique(s): microarray: suitable; western blot: 1 μg/mL using total cell extracts of NIH3T3 cells
• isotype: IgM
• UniProt accession no.: O15379
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... HDAC3(8841) ; mouse ... Hdac3(15183)

Description

General description

Monoclonal Anti-Histone Deacetylase 3 (HDAC3) (mouse IgM isotype) is derived from the HDAC3-83 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. The histone deacetylase 3 (HDAC3) gene is located on the human chromosome at 5q31.3. HDAC3 protein belongs to the class I HDAC.

Specificity

Monoclonal Anti-Histone Deacetylase 3 (HDAC3) recognizes human and mouse HDAC3 (approx. 50 kDa).

Immunogen

synthetic peptide corresponding to amino acids 411-428 of human and mouse HDAC3.

Application

Monoclonal Anti-Histone Deacetylase 3 (HDAC3) antibody produced in mouse may be used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Biochem/physiol Actions

Histone deacetylase 3 (HDAC3) protein is required for DNA replication, maintaining chromatin structure, genomic stability, and DNA damage control. This protein plays a role in eliminating histone acetylation. HDAC3 protein co-works with nuclear hormone corepressors, such as nuclear receptor corepressor (NCOR) and silencing mediator of retinoic acid and thyroid hormone receptor (SMRT). HDAC3 protein levels are high in proliferating cells of hepatocellular carcinoma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.