H5539
Anti-HUS1, C-terminal antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 32 kDa
species reactivity
human
technique(s)
western blot: 1:250-1:500 using HeLa cell nuclear extracts or K562 cell extracts
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HUS1(3364)
General description
HUS1 checkpoint protein is a part of the Hus1-Rad1-Rad9 heterotrimeric complex and forms a PCNA-ring-like structure. The HUS1 gene is mapped on the human chromosome at 7p12.3.
Specificity
Anti-Hus1, C-terminal, specifically recognizes Hus1.
Immunogen
synthetic peptide corresponding to amino acids 253-269 of human Hus1, conjugated to KLH via an N-terminal cysteine residue.
Application
Anti-HUS1, C-terminal antibody produced in rabbit may be used in immunoblotting.
Biochem/physiol Actions
HUS1 protein along with cell cycle checkpoint proteins Rad1 and Rad9 is involved in cell cycle arrest as a response to DNA damage and acts as a sliding clamp. Upregulation of HUS1 gene expression leads to inhibition of cell proliferation, migration, invasion, and cell cycle arrest in hepatocellular carcinoma (HCC).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
related product
Product No.
Description
Pricing
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Swapnil Ulhas Rane et al.
Breast cancer research and treatment, 153(1), 101-121 (2015-08-10)
Ductal carcinoma in situ (DCIS) is a pre-invasive malignancy detected with an increasing frequency through screening mammography. One of the primary aims of therapy is to prevent local recurrence, as in situ or as invasive carcinoma, the latter arising in
R Kaur et al.
Molecular biology of the cell, 12(12), 3744-3758 (2001-12-12)
Hus1, Rad1, and Rad9 are three evolutionarily conserved proteins required for checkpoint control in fission yeast. These proteins are known to form a stable complex in vivo. Recently, computational studies have predicted structural similarity between the individual proteins of Hus1-Rad1-Rad9
Zi-Qi Zhou et al.
Molecular carcinogenesis, 58(1), 76-87 (2018-09-06)
The HUS1 checkpoint clamp component (HUS1), which is a member of an evolutionarily conserved, genotoxin-activated checkpoint complex (Rad9-Rad1-Hus1 [9-1-1] complex), is involved in cell cycle arrest and DNA repair in response to DNA damage. We conducted this study to investigate
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
