H4664

Anti-HUS1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• MDL number: MFCD08705464
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 32 kDa
• species reactivity: human
• technique(s): immunoprecipitation (IP): 2.5-5 μg using HeLa cell lysates; western blot: 1-2 μg/mL using HeLa nuclear extracts
• UniProt accession no.: O60921
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... HUS1(3364)

Description

General description

HUS1 checkpoint protein is a member of the genotoxin-activated checkpoint (Hus1-Rad1-Rad9) complex. The HUS1 gene is mapped on the human chromosome at 7p12.3.

Specificity

Anti-HUS1 specifically recognizes human HUS1.

Immunogen

synthetic peptide corresponding to amino acids 147-164 of human HUS1, conjugated to KLH via an N-teminal added cysteine residue.

Application

Anti-HUS1 antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

HUS1 checkpoint protein is involved in DNA repair and cell cycle arrest as a result of DNA damage. Upregulation of HUS1 gene expression is associated with the inhibition of cell proliferation, migration, invasion, and cell cycle arrest in hepatocellular carcinoma (HCC). Loss of HUS1 gene leads to embryonic lethality.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)