H4042

HGH active human

Animal-component free, recombinant, expressed in Nicotiana, >97% (SDS-PAGE)

• Synonym(s): GH, GH-N, Growth hormone, Growth hormone 1, Pituitary growth hormone
• UNSPSC Code: 51111800

Properties

• biological source: human
• recombinant: expressed in Nicotiana
• assay: >97% (SDS-PAGE)
• form: lyophilized
• potency: ≤0.1 ng/mL
• mol wt: 22.9 kDa (205 amino residues)
• packaging: pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: Endotoxin, tested (LAL test, < 0.04 EU/ ug protein)
• UniProt accession no.: P01241
• storage temp.: −20°C
• Gene Information: human ... GH1(2688)

Description

General description

GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control.

GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.

Biochem/physiol Actions

GH (growth hormone) is mainly crucial at puberty for the mammary gland development. In epithelioid breast cancer, autocrine GH enhances epithelial-mesenchymal transition. In addition, GH is needed for lipid metabolism and adipose tissue remodelling.

Physical form

Lyophilized from a PBS 0.05 M buffer at pH 7.5

Reconstitution

Lyophilized protein should be reconstituted in water to a concentration of 50 ng/μl. Due to the protein nature, dimmers and multimers may be observed.

Analysis Note

The biological activity of human Growth Hormone is measured by cell proliferation using Nb2-11 cells.
ED₅₀ ≤ 0.04-0.1 ng/mL

Other Notes

Extinction Coefficient: E^0.1% = 0.77 (A₂₈₀ nm)
p.I: 5.98

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 2