H4038

Anti-Heat Shock Protein 40 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-HSP40
• MDL number: MFCD02263107
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~35 kDa
• species reactivity: mouse, human
• technique(s): western blot: 1-2 μg/mL using total extracts of NIH3T3-L1 cells
• UniProt accession no.: P25685
• shipped in: dry ice
• Storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DNAJB1(3337) ; mouse ... Dnajb1(81489)

Description

General description

Cells express an increased amount of several highly conserved proteins referred to as heat shock or heat stress proteins (Hsps). They are primarily found in the cytoplasm, nucleus, and mitochondria. Mammalian cells Hsps are classified into several families of sequence-related proteins, which are differentiated by their molecular sizes. Major families are as follows: the Hsp25/Hsp27/Hsp28 family (the small stress proteins), the Hsp40 family, the Hsp60 family, the Hsp70 family, the Hsp90 family, and the Hsp110/SSE family. HSP40, also known as DNAJB1, HSPF1, or HDJ1, is a 339-amino acid, stress inducible heat shock protein, that is homologous to the bacterial heat shock protein DnaJ, and to the yeast DnaJ-related proteins such as SCJ1, Sec63/Np11, YDJ1, and SIS1.

Heat Shock Protein 40 (HSP40) is a 40 kDa, stress inducible heat shock protein, that is homologous to the bacterial heat shock protein DnaJ, and to the yeast DnaJ-related proteins.

Immunogen

synthetic peptide corresponding to amino acids 323-339 of human HSP40, conjugated to KLH via an N-terminal added lysine residue. The peptide sequence differs from mouse in three amino acids.

Application

Anti-Heat Shock Protein 40 antibody produced in rabbit has been used in western blotting and microarray.

Biochem/physiol Actions

In heat-shocked cells, Hsp40 translocates from the cytoplasm to the nucleus and nucleoli, colocalizing with Hsp70. Hsp40 and Hsp70 have been implicated in reduction of protein aggregates produced in neurodegenerative diseases, such a Huntington′s and Parkinson′s diseases. In brain tissues from Parkinson disease patients, it has been shown that Lewy bodies (LBs) and Lewy neurites (LNs) are immunopositive for Hsp70 and Hsp40 chaperones, suggesting that altered chaperone activity may be involved in progression of Parkinson disease.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)