Both products are peptide hydrogels. There is no similarity between the peptides used to create the matrices. They are both just classified as self-assembling peptide hydrogels. The sequence for the peptide is considered proprietary.
H3915
HydroMatrix Peptide Cell Culture Scaffold
6 well plate
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About This Item
UNSPSC Code:
41116155
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Application
HydroMatrix has been shown to support improved growth and migration in the proliferation of many cell types, including neural stem cells, neurons, glia, astrocytes, fibroblasts, and keratinocytes.
Components
HydroMatrix is a peptide nanofiber 3-dimensional scaffold that promotes cell growth and migration. It utilizes specific peptides that self-assemble from fluid precursors into highly crosslinked peptide hydrogels when presented with increased temperatures or ionic strengths. By inducing a rapid transformation from solution to gel, a peptide nanofiber scaffold is generated. Adjusting the concentration of the HydroMatrix solution, researchers can control the flexibility of the 3-D architecture to meet their application′s specific needs.
Caution
The lyophilized product at -20°C and is stable for many years at this temperature. The hydrogel should be stored at -70°C to maximize its active span. The 1% (w/v) stock solution should be stored -20°C and is stable for 3-6 months.
Preparation Note
This product is supplied as a lyophilized powder. A 1% (w/v) stock solution at 10 mg/mL can be prepared with addition of sterile water, resulting in a rigid gel. A 0.5% (w/v) solution will lead to a softer gel that is more suitable for cell migration and angiogenesis. Lower concentrations in the range of 0.15%-0.25% are more suitable for many cell types including neurons. In aqueous solution, HydroMatrix has a pH of 2.5 and should not be added directly to cells without prior addition of a buffer or medium. Once the hydrogel has formed, do not attempt to mix the gel any further, as this risks destroying the hydrogel integrity. Manipulation of the cells should be carefully performed to avoid disruption of the scaffold.
Legal Information
Storage Class
13 - Non Combustible Solids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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How does HydroMatrix™ Peptide Cell Culture Scaffold compare to PuraMatrix from Becton-Dickenson?
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How do I store HydroMatrix™ Peptide Scaffold, Product H3915?
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Store the product at -20°C as supplied. Once the material is reconstituted, to a 1% (w/v) (10 mg/ml) stock solution, with dH2O, the stock solution should be stored at -20°C and is stable for 3-6 months.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How is HydroMatrix™ Peptide Scaffold, Product H3915, supplied?
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This product is supplied in the plate in a lyophilized form. For the 6 well plate to achieve a 1% w/v (10 mg/ml stock concentration), reconstitute with H2O at a volume of 2.4 ml per well.
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How thick is the layer on the HydroMatrix™ Peptide Scaffold, Product H3915, plate?
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We have not determined the actual thickness of the layer, but it is thick enough to coat the bottom of the well.
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How do I plate my cells on the HydroMatrix™ Peptide Scaffold, Product H3915, plates?
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Determine the optimal concentration for your application and dilute the stock with dH2O. Keep the diluted solution on ice until ready to use. Induce formation of the gel by adding 1-2 volumes of medium to the side of the well. Incubate the plate at 37°C to allow gel to form. Change the media 1-2 times over 1-2 hours and allow the gel to incubate at 37°C during this time. Keep the gelled plate at 37°C for no more than 8 hours before use. Add desired number of cells in media to top of the HydroMatrix™.
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