immunoprecipitation (IP): 5-10 μg using RIPA lysate of human HeLa cells indirect immunofluorescence: 5-10 μg/mL using mouse NIH3T3 cells western blot (chemiluminescent): 1-2 μg/mL using whole extract of human HeLa cells
synthetic peptide corresponding to amino acid residues 177-191 of human HP1a with N-terminal added cysteine, conjugated to KLH. The corresponding sequence in mouse differs by one amino acid.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
The trimethylation of histone H3 on lysine 9 (H3K9me3) - a mark recognized by HP1 that depends on the Suv39h lysine methyltransferases (KMTs) - has provided a basis for the reader/writer model to explain HP1 accumulation at pericentric heterochromatin in
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We present a simple ratio method to infer protein composition within cellular structures using proximity labeling approaches but compensating for the diffusion of free radicals. We used tyramide signal amplification (TSA) and label-free mass spectrometry (MS) to compare proteins in
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