H17002
LIF (Leukemia Inhibitory Factor), human
recombinant, expressed in HEK 293 cells
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About This Item
UNSPSC Code:
12352202
recombinant
expressed in HEK 293 cells
assay
>95% (SDS-PAGE)
potency
≤0.2 ng/mL EC50
mol wt
33-48 kDa (glycosylated)
impurities
<1 EU/μg endotoxin
storage temp.
−20°C
General description
LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation.
Reconstitution
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1X PBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).
Storage and Stability
Upon reconstitution, the cytokine can be stored at 4°C for short term only or at -20°C to -80°C in aliquots for long term. Avoid repeated freeze-thaw cycles.
Analysis Note
The activity was determined by the dose- dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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