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H1290

Sigma-Aldrich

HIV Protease Mutant

recombinant, expressed in E. coli

Synonym(s):

(L33F, M46L, I54V, V82A, L90M, L10I, M36I, A71V, L63P)

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About This Item

EC Number:
3.4.23.16 (BRENDA, IUBMB)
UNSPSC Code:
12352204

recombinant

expressed in E. coli

form

solution

mol wt

~10.8 kDa

shipped in

dry ice

storage temp.

−70°C

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Features and Benefits

Stabilized without non-physiologic mutations

Packaging

100 unit vial containing ≥ 100ug protein

Unit Definition

One unit of HIV protease will cleave 1picomole per minute of the substrate Abz-Ala-Arg-Val-Nle-Tyr(NO2)-Glu-Ala-Nle-NH2 at 37°C.

Physical form

Supplied as a solution in 0.5M Sodium Acetate, pH 4.7with 50mM Tris-HCL, 1M NaCL, 1mM EDTA, 5mM DTT and 15% glycerol.

Preparation Note

Bioengineered to resist autoproteolysis, resulting in an enzyme with improved stability and substantially higher activity levels over extended time periods to conduct kinetic assays. The enzyme includes the most commonly occurring background polymorphism (L63P) that aids in the drug resistance profile.

substrate

Product No.
Description
Pricing

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
088K1762

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