WDR4 (WD repeat domain 4) is a member of the WD repeat protein family, and the gene is localized to human chromosome 21q22.3. This gene is composed of 11 exons, and spans 37kb. Alternative splicing results in two RNA variants of sizes 1.5kb and 2.1kb, where the smaller one is predominantly expressed in fetal tissues. This protein contains four guanine nucleotide-binding WD repeats, and is composed of 412 amino acids. It is the human homologue of TRM82 (tRNA methylase), a protein found in Saccharomyces cerevisiae.
Anti-WDR4 antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.
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WDR4 (WD repeat domain 4) is mapped between PDE9A and NDUFV3, a region where several genetic disorders are mapped. It forms a protein complex along with Trm8. WDR4 is required for m7G-methyltransferase activity.
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Solution in phosphate buffered saline containing 0.02% sodium azide.
To identify candidate genes for Down syndrome phenotypes or disorders that map to human chromosome 21q22.3, trapped exons are being used to isolate full-length transcripts. We isolated a full-length cDNA (WDR4) encoding a novel WD-repeat protein and its mouse homologue.
A substrate for protein kinase B (PKB)alpha in HeLa cell extracts was identified as methyltransferase-like protein-1 (METTL1), the orthologue of trm8, which catalyses the 7-methylguanosine modification of tRNA in Saccharomyces cerevisiae. PKB and ribosomal S6 kinase (RSK) both phosphorylated METTL1
RNA (New York, N.Y.), 8(10), 1253-1266 (2002-10-31)
7-methylguanosine (m7G) modification of tRNA occurs widely in eukaryotes and bacteria, is nearly always found at position 46, and is one of the few modifications that confers a positive charge to the base. Screening of a Saccharomyces cerevisiae genomic library
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