GW21421
Anti-MITF antibody produced in chicken
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-Microphthalmia-associated transcription factor
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
chicken
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MITF(4286)
General description
MITF (microphthalmia-associated transcription factor) is a b-HLH-Zip (basic helix-loop-helix leucine zipper) transcription factor, which is specific to melanocytes. It is a member of the Myc superfamily, and the gene is localized to human chromosome 3. It is 229kb in size. It belongs to the MiT (microphthalmia) family of transcription factors, which also includes TFEB (transcription factor EB), TFEC and TFE3. Due to alternative splicing, this protein is produced as multiple different isoforms such as TF-A, MITF-B, MITF-C, MITF-D, MITF-E, MITF-H, MITF-J, MITF-M and MITF-Mc. The N-termini of these isoforms vary, and they have different tissue expression patterns. The shortest isoform is MITF-M, which is only expressed in melanoma cells and melanocytes. Its transcriptional activity is modulated by its two TADs (transactivation domains), present at N- and C-termini.
Immunogen
Immunogen Sequence: GI # 4557755, sequence 287-417
Recombinant microphthalmia-associated transcription factor isoform 4
Application
Anti-MITF antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.
Biochem/physiol Actions
Microphthalmia-associated transcription factor (MITF) is involved in lineage-specific pathway regulation of many types of cells. Mitf is required for the proper development of osteoclasts, melanocytes, retinal pigment epithelial cells, mast cells and natural killer cells. It can be a valuable adjunct in the diagnosis of metastatic tumors that are suspicious for melanoma but negative for other melanoma markers.
Physical form
Solution in phosphate buffered saline containing 0.02% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Ruifeng Guo et al.
International journal of clinical and experimental pathology, 6(8), 1658-1664 (2013-08-08)
Metastatic malignant melanoma has a wide spectrum of histopathologic patterns and often lacks melanin pigment. Without a known primary tumor, the diagnosis of metastatic malignant melanoma relies on a combination of morphology and immunohistochemical profile. Infrequently, commonly used markers for
Mariusz L Hartman et al.
Cellular and molecular life sciences : CMLS, 72(7), 1249-1260 (2014-12-01)
MITF (microphthalmia-associated transcription factor) represents a melanocytic lineage-specific transcription factor whose role is profoundly extended in malignant melanoma. Over the last few years, the function of MITF has been tightly connected to plasticity of melanoma cells. MITF participates in executing
Christine L Hershey et al.
Bone, 34(4), 689-696 (2004-03-31)
The Microphthalmia-associated transcription factor (Mitf) is required for the proper development of several cell lineages including osteoclasts, melanocytes, retinal pigment epithelial cells, mast cells and natural killer cells. Mutations in Mitf in multiple organisms result in osteopetrosis due to defective
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