GW20092F
Anti-Prothrombin antibody produced in chicken
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-Coagulation factor II, Anti-PT
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
chicken
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
human
manufacturer/tradename
Genway 15-288-20092F
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... F2(2147)
General description
Coagulation factor II (F2) is the precursor of thrombin belonging to serine protease family. It is mainly involved in the coagulation cascade i.e. hemostasis and thrombosis.
Application
Anti-Prothrombin antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.
Biochem/physiol Actions
Thrombin is a serine protease encoded by the F2 gene in humans. Prothrombin (coagulation factor II) is proteolytically cleaved to form thrombin in the coagulation cascade and ultimately results in the reduction of blood loss. It is generated at the sites of vascular injury and acts as a potent activator of platelets. It activates platelets as well as the blood coagulation factors V, VIII and XIII, which plays an essential role in blood clot mechanism. Moreover, proteolysis of fibrinogen by thrombin generates fibrin monomers which undergoes polymerization and form the basis of this clot. The activity of thrombin is inhibited by hirudin by the formation of a tight, non-covalent complex. It has been shown that a region adjacent to the proteolytic pocket of thrombin confers high-affinity binding capacity which helps in generation of mitogenic signals. Its mutation is implicated as a risk factor for venous thrombosis, its role in arterial ischemic stroke is not known, especially among young adults.
Physical form
Solution in phosphate buffered saline containing 0.02% sodium azide.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Baijia Jiang et al.
Stroke, 45(4), 961-967 (2014-03-13)
Although the prothrombin G20210A mutation has been implicated as a risk factor for venous thrombosis, its role in arterial ischemic stroke is unclear, particularly among young adults. To address this issue, we examined the association between prothrombin G20210A and ischemic
Ping He et al.
International journal of hematology, 110(1), 59-68 (2019-04-22)
Bleeding into the joints represents the major morbidity of severe hemophilia and predisposes it to hemophilic arthropathy (HA). In a reproducible hemarthrosis mouse model, we found distinct changes in thrombin activity in joint tissue homogenate following exposure of the joint
Reliable genotyping of the G-20210-A mutation of coagulation factor II (prothrombin).
J Danneberg et al.
Clinical chemistry, 44(2), 349-351 (1998-02-25)
K C Glenn et al.
Peptide research, 1(2), 65-73 (1988-11-01)
Initiation of cell proliferation by thrombin requires signals generated by thrombin interaction with specific high-affinity receptors and thrombin enzymic activity. Using synthetic peptides representing various domains of thrombin, we have identified a region adjacent to the proteolytic pocket of thrombin
J M Herbert et al.
The Biochemical journal, 303 ( Pt 1), 227-231 (1994-10-01)
Binding of 125I-thrombin to human umbilical vein endothelial cells (HUVECs) was specifically displaced by the synthetic tetradecapeptide SFLLRNPNDKYEPF, named thrombin receptor agonist peptide (TRAP), which has recently been described as a peptide mimicking the new N-terminus created by cleavage of
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service