Recommended Products
manufacturer/tradename
Cytiva RPN2634
packaging
pkg of 96 wells
shipped in
dry ice
Storage temp.
−20°C
General description
Biotrak™ MMP-9, Activity Assay System Companies provides a simple, specific and precise quantitative determination of active or pro MMP-9 in tissue culture supernatants, serum, plasma, synovial fluid and tissue homogenates. Active MMP-9 may be measured in the range 0.5 to 16 ng/mL for normal endogenous MMP-9 levels. A longer incubation protocol enables an assay range of 0.125 to 4 ng/mL for lower endogenous MMP-9 levels. The assay uses the pro form of a detection enzyme that can be activated by captured active MMP-9, into an active detection enzyme, through a single proteolytic event. The natural activation sequence in the pro detection enzyme has been replaced using protein engineering, with an artificial sequence recognized by specific matrix metalloproteinases.
MMP activated detection enzyme can then be measured using a specific chromogenic peptide substrate. Standards and samples are incubated in microtitre wells precoated with anti-MMP-9 antibody. Any MMP-9 present will be bound to the wells, other components of the sample being removed by washing and aspiration. Either the endogenous levels of free active MMP-9, or the total levels of free MMP-9 in a sample, can be detected. In order to measure the total MMP-9 content, any bound MMP-9 in its pro form is activated using p-Aminophenylmercuric Acetate (APMA). The standard is pro MMP-9 which is activated in parallel for both types of sample. Active MMP- 9 is detected through activation of the modified pro detection enzyme and the subsequent cleavage of its chromogenic peptide substrate. The resultant colour is read at 405 nm in a microplate spectrophotometer. The concentration of active MMP-9 in a sample is determined by interpolation from a standard curve. Each pack contains reagents for 96 determinations. This allows the construction of a standard curve plus the measurement of 42 samples in duplicate. The Biotrak™ MMP-9 activity assay system has been specifically designed for research purposes.
MMP activated detection enzyme can then be measured using a specific chromogenic peptide substrate. Standards and samples are incubated in microtitre wells precoated with anti-MMP-9 antibody. Any MMP-9 present will be bound to the wells, other components of the sample being removed by washing and aspiration. Either the endogenous levels of free active MMP-9, or the total levels of free MMP-9 in a sample, can be detected. In order to measure the total MMP-9 content, any bound MMP-9 in its pro form is activated using p-Aminophenylmercuric Acetate (APMA). The standard is pro MMP-9 which is activated in parallel for both types of sample. Active MMP- 9 is detected through activation of the modified pro detection enzyme and the subsequent cleavage of its chromogenic peptide substrate. The resultant colour is read at 405 nm in a microplate spectrophotometer. The concentration of active MMP-9 in a sample is determined by interpolation from a standard curve. Each pack contains reagents for 96 determinations. This allows the construction of a standard curve plus the measurement of 42 samples in duplicate. The Biotrak™ MMP-9 activity assay system has been specifically designed for research purposes.
Features and Benefits
- Specific for MMP-9.
- Measures endogenous active MMP-9 or total active MMP-9 (following activation using APMA).
- Nonradioactive microplate-based assay.
- Precise and accurate measurement.
- Applicable to complex biological samples.
- Ready-to-use detection enzyme and substrate.
- Equivalent sensitivity to ELISA
Storage and Stability
Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Biotrak MMP-1, MMP-3, MMP-9, and TIMP-1 Human ELISAs
These products are developed in collaboration with FUJI CHEMICAL INDUSTRIES, Ltd.(Japan).
These products are developed in collaboration with FUJI CHEMICAL INDUSTRIES, Ltd.(Japan).
Biotrak is a trademark of Cytiva
signalword
Danger
Storage Class
6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.
If you need assistance, please contact Customer Support.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
