GERPN2614
Matrix Metalloproteinase-9 (MMP-9) Human, Biotrak™ Assay
Cytiva RPN2614, pack of 1 ea, size 96 wells
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
packaging
pack of 1 ea
manufacturer/tradename
Cytiva RPN2614
size
96 wells
shipped in
dry ice
storage temp.
−20°C
General description
The Biotrak™ MMP-9 ELISA provides a simple, specific, and precise quantitative determination of MMP-9 in tissue culture supernatants and homogenates as well as plasma.
Application
The assay is based on a two-site ELISA sandwich format using two antibodies directed against different epitopes of MMP-9. During the first incubation step, MMP-9 present in the samples or the standards is bound to a microplate pre-coated with antibody. During the second incubation step, detection antibody conjugated to horseradish peroxidase is added, which forms an immobilized complex. The amount of peroxidase bound to each well is determined by the addition of tetramethylbenzidine (TMB) ‘ready-to-use′ substrate. The reaction is stopped by the addition of an acid solution and the resultant color measured at 450 nm in a microplate spectrophotometer. The concentration of proMMP-9 in samples is determined by interpolation from a standard curve. MMP-9 may be measured in the range 1–32 ng/mL for tissue culture and the sensitivity of the assay is 0.6 ng/mL.
Each pack contains sufficient material for 96 wells. This permits the construction of one standard curve and measurement of 40 unknowns in duplicate. A separate protocol is included for the measurement of MMP-9 in plasma (assay range 4–128 ng/mL). The Biotrak™ MMP-9 ELISA is suitable for research purposes only.
Each pack contains sufficient material for 96 wells. This permits the construction of one standard curve and measurement of 40 unknowns in duplicate. A separate protocol is included for the measurement of MMP-9 in plasma (assay range 4–128 ng/mL). The Biotrak™ MMP-9 ELISA is suitable for research purposes only.
Features and Benefits
- High Sensitivity.
- Simple, convenient & rapid.
- Low background.
- Non-isotopic protocol.
- Ready for use substrate.
- Same day protocol.
Storage and Stability
Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Biotrak MMP-1, MMP-3, MMP-9, and TIMP-1 Human ELISAs
These products are developed in collaboration with FUJI CHEMICAL INDUSTRIES, Ltd.(Japan).
These products are developed in collaboration with FUJI CHEMICAL INDUSTRIES, Ltd.(Japan).
Biotrak is a trademark of Cytiva
Storage Class
13 - Non Combustible Solids
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.
If you need assistance, please contact Customer Support.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
