GERPN2610
MMP-1, Human, Biotrak™ ELISA
Cytiva RPN2610, pack of 1 ea, size 96 wells
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
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packaging
pack of 1 ea
manufacturer/tradename
Cytiva RPN2610
size
96 wells
shipped in
dry ice
storage temp.
−20°C
General description
MMP-1, Human, Biotrak™ Assay.
Application
The MMP-1 Human Biotrak™ ELISA system has been specifically designed for research purposes. MMP-1 may be measured in the range 6.25-100 ng/mL.
The assay is based on a two site ELISA ‘sandwich′ format. Standards and samples are incubated in microplate wells pre-coated with anti-MMP-1 antibody. Any MMP-1 present will be.
bound to the wells, other components of the sample being removed by washing and aspiration. A second polyclonal antibody to MMP-1 is then bound to the MMP-1. Any excess is removed by washing and aspiration. The second antibody bound to the wells is detected using donkey anti-rabbit horseradish peroxidase. The amount of peroxidase bound to each well is determined by the addition of TMB ‘ready-to-use′ substrate. The reaction is stopped by addition of an acid solution, and the resultant color read at 450 nm in a microplate spectrophotometer. The concentration of MMP-1 in a sample is determined by interpolation from a standard curve.
Each pack contains reagent for 96 determinations. This allows the construction of a standard curve plus the measurement of 42 samples in duplicate.
The assay is based on a two site ELISA ‘sandwich′ format. Standards and samples are incubated in microplate wells pre-coated with anti-MMP-1 antibody. Any MMP-1 present will be.
bound to the wells, other components of the sample being removed by washing and aspiration. A second polyclonal antibody to MMP-1 is then bound to the MMP-1. Any excess is removed by washing and aspiration. The second antibody bound to the wells is detected using donkey anti-rabbit horseradish peroxidase. The amount of peroxidase bound to each well is determined by the addition of TMB ‘ready-to-use′ substrate. The reaction is stopped by addition of an acid solution, and the resultant color read at 450 nm in a microplate spectrophotometer. The concentration of MMP-1 in a sample is determined by interpolation from a standard curve.
Each pack contains reagent for 96 determinations. This allows the construction of a standard curve plus the measurement of 42 samples in duplicate.
Features and Benefits
- Measures total MMP-1.
- Specific for human MMP-1.
- Precise and accurate measurement.
- Non-isotopic protocol.
- Ready to use substrate.
- Same day protocol.
Storage and Stability
Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Biotrak is a trademark of Cytiva
Storage Class
12 - Non Combustible Liquids
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