GERPN224
Leukotriene C4/D4/E4, Biotrak™ EIA System
Cytiva RPN224
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
manufacturer/tradename
Cytiva RPN224
packaging
pkg of 96 wells
shipped in
dry ice
storage temp.
−20°C
General description
Leukotriene C4/D4/E4 Biotrak™ enzymeimmunoassay system from Cytiva has been specifically designed for research purposes. It combines the use of a Peroxidase labelled Leukotriene C4 conjugate, a peptido Leukotriene specific antiserum which can be immobilized on to pre-coated microplates, and a one pot stabilized substrate solution. This provides a rapid and sensitive non-isotopic method for the determination of Leukotriene C4/D4/E4 in the range 0.77 pg to 49.5 pg/well. Each pack contains sufficient material for 96 wells. This permits the construction of one standard curve and 39 unknowns in duplicate. The assay is based on the competition between unlabelled Leukotriene C4 and a fixed quantity of Peroxidase labelled Leukotriene C4 for a limited number of binding sites on a peptido leukotriene specific antibody.
With fixed amounts of antibody and Peroxidase labelled Leukotriene C4 the amount of Peroxidase labelled ligand bound by the antibody will be inversely proportional to the concentration of added unlabelled ligand. The Peroxidase ligand that is bound to the antibody is immobilized on to polystyrene microplate wells precoated with second antibody, as demonstrated in figure 1. Thus any unbound ligand can be removed from the well by simple washing procedures. The amount of Peroxidase labelled Leukotriene C4 bound to the antibody is determined by addition of Tetramethylbenzidine (TMB)/ Hydrogen Peroxide single pot substrate. The reaction is stopped by addition of an acid solution, and the resultant colour read at 450 nm in a microplate spectrophotometer. The concentration of unlabelled Leukotriene C4 in a sample is determined by interpolation from a standard curve.
With fixed amounts of antibody and Peroxidase labelled Leukotriene C4 the amount of Peroxidase labelled ligand bound by the antibody will be inversely proportional to the concentration of added unlabelled ligand. The Peroxidase ligand that is bound to the antibody is immobilized on to polystyrene microplate wells precoated with second antibody, as demonstrated in figure 1. Thus any unbound ligand can be removed from the well by simple washing procedures. The amount of Peroxidase labelled Leukotriene C4 bound to the antibody is determined by addition of Tetramethylbenzidine (TMB)/ Hydrogen Peroxide single pot substrate. The reaction is stopped by addition of an acid solution, and the resultant colour read at 450 nm in a microplate spectrophotometer. The concentration of unlabelled Leukotriene C4 in a sample is determined by interpolation from a standard curve.
Features and Benefits
- High sensitivity ~ 0.5 pg/well for Leukotriene C4/D4, 10 pg/mL.
- Nonisotopic.
- Ready-to-use substrate.
- Color-coded reagents.
- Choice of protocol.
Storage and Stability
Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Biotrak is a trademark of Cytiva
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
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