GERPN220
Thromboxane B2, Biotrak™ EIA
Cytiva RPN220, pack of 1 ea, size 96 wells
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About This Item
UNSPSC Code:
12352200
packaging
pack of 1 ea
manufacturer/tradename
Cytiva RPN220
size
96 wells
storage temp.
2-8°C
General description
Thromboxane B?, Biotrak™ Assay.
Application
Biotrak™ thromboxane B2 enzymeimmunoassay system has been specifically designed for research purposes. It combines the use of a peroxidase labelled thromboxane B2 conjugate, a specific antiserum which can be immobilized on to pre-coated microplates, and a one pot stabilized substrate solution. This provides a rapid and sensitive non-isotopic method for the determination of thromboxane B2 in the range 0.5 to 64 pg/well. Each pack contains sufficient material for 96 wells. This permits the construction of one standard curve and 37 unknowns in duplicate.
The assay is based on the competition between unlabeled thromboxane B2 and a fixed quantity of peroxidase-labeled thromboxane B2 for a limited number of binding sites on a thromboxane B2-specific antibody. With fixed amounts of antibody and peroxidase-labeled thromboxane B2, the amount of peroxidase- labeled ligand bound by the antibody will be inversely proportional to the concentration of added unlabeled ligand. The peroxidase ligand that is bound to the antibody is immobilized on to polystyrene microplate wells precoated with second antibody. Therefore, any unbound ligand can be removed from the well by simple washing procedures. The amount of peroxidase labelled thromboxane B2 bound to the antibody is determined by addition of a tetramethylbenzidine (TMB)/ hydrogen peroxide single pot substrate. The reaction is stopped by additon of an acid solution, and the resultant colour read at 450 nm in a microplate photometer. The concentration of unlabelled thromboxane B2 in a sample is determined by interpolation from a standard curve.
The assay is based on the competition between unlabeled thromboxane B2 and a fixed quantity of peroxidase-labeled thromboxane B2 for a limited number of binding sites on a thromboxane B2-specific antibody. With fixed amounts of antibody and peroxidase-labeled thromboxane B2, the amount of peroxidase- labeled ligand bound by the antibody will be inversely proportional to the concentration of added unlabeled ligand. The peroxidase ligand that is bound to the antibody is immobilized on to polystyrene microplate wells precoated with second antibody. Therefore, any unbound ligand can be removed from the well by simple washing procedures. The amount of peroxidase labelled thromboxane B2 bound to the antibody is determined by addition of a tetramethylbenzidine (TMB)/ hydrogen peroxide single pot substrate. The reaction is stopped by additon of an acid solution, and the resultant colour read at 450 nm in a microplate photometer. The concentration of unlabelled thromboxane B2 in a sample is determined by interpolation from a standard curve.
Features and Benefits
- High sensitivity ~ 0.2 pg.
- 2 h protocol.
- Nonisotopic.
- Ready-to-use substrate.
- Color-coded reagents.
Storage and Stability
Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Biotrak is a trademark of Cytiva
Storage Class
11 - Combustible Solids
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