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GE29-0362-25

Superose® 12 3.2/300

Cytiva 29-0362-25, column L × I.D. 30 cm × 3.2 mm, 11 μm avg. part. size

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About This Item

UNSPSC Code:
41115700

material

borosilicate glass column
titanium frit

mol wt

1000-300000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 29-0362-25

availability

not available in USA, Canada and Puerto Rico

parameter

0.01-0.1 mL/min flow rate
2.4 Mpa max. pressure (350 psi)
4-50 mL sample volume

column L × I.D.

30 cm × 3.2 mm

matrix active group

agarose phase

avg. part. size

11 μm

working range

3-12

separation technique

size exclusion (SEC)

General description

The Precision Column PC 3.2/30 (2.4 ml), prepacked with Superose12, is designed for sensitive and highly resolving gel filtration separations of biomolecules in the micropreparative scale. Superose 12 has a broad fractionation range for molecular weights between 1000 and 300 000.

Features and Benefits

  • Achieve high-resolution separations across exceptionally broad molecular-weight ranges with Superose gel filtration medium.
  • Precision columns (PC) for micropurification and analysis: sample volumes of 4 - 50 μL.
  • Low ionic strength eluents (< 0.05 M) can utilize a hydrophobic interaction component to alter the selectivity of Superose for some lipids, peptides, and small aromatic compounds.
  • New hardware design for convenient direct connection to high performance chromatography systems such as ÄKTA design.

Suitability

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place). Columns are not suitable for use with ÄKTAprime plus system.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superose is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

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