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GE28-9374-51

DIGE Gel

Cytiva 28-9374-51, pack of 3 pieces

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About This Item

UNSPSC Code:
41105323
NACRES:
NA.25

form

solid

feature

wetted part: no

packaging

pack of 3 pieces

manufacturer/tradename

Cytiva 28-9374-51

storage temp.

2-8°C

General description

DIGE Gel and DIGE Buffer Kit are tools to increase the quality, reproducibility, and convenience of 2-D DIGE using Ettan DIGE system. DIGE gel is a 12.5% precast polyacrylamide gel in a low fluorescent glass cassette for 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE) and specially developed for 2-D DIGE analysis. The DIGE Buffer Kit is a specially designed two-buffer system for running up to twelve DIGE gels simultaneously.

DIGE Gel should be used with the DIGE Buffer Kit, which consists of concentrated running buffers and sealing solution for attaching Immobiline DryStrip Gels (IPG Strips) to the top of the polyacrylamide gel. The capacity of the buffer system is similar to the commonly used laemmli (Tris-Glycine) buffer system, and the separation performance of DIGE Gel is comparable to 12.5% laemmli gels (1). DIGE Gel and DIGE Buffer Kit offer the high quality and performance of the laemmli system with the added advantages of convenience and time savings.

DIGE Gel is intended for use with the DIGE Buffer Kit and Ettan DALT large vertical system. Ettan DALTsix is designed for large-format, second dimension electrophoresis using either 18 or 24 cm Immobiline DryStrip Gels. The DIGE Buffer Kit contains the amount of reagents necessary for two runs of up to six gels using the Ettan DALTsix unit.

Features and Benefits

  • Precast 12.5% polyacrylamide gel and buffer for 2-D DIGE.
  • Ready-to-use products with a long shelf-life that saves you time by eliminating the need to make buffers, cast gels, and wash glass plates, leaving time for more important work.
  • DIGE Gels provide high sensitivity and high resolution protein spot maps of equal quality compared to homecast polyacrylamide gels.
  • DIGE Gels, run together with DIGE Buffer Kit, results in spot maps with higher reproducibility compared to homecast polyacrylamide gels.

Storage and Stability

Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 2


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Certificates of Analysis (COA)

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Protocols

This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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