GE28-4082-53

HiTrap^® MabSelect^™

Cytiva 28-4082-53, pack of 5 × 1 mL

• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: recombinant protein A (E. coli)
• packaging: pack of 5 × 1 mL
• manufacturer/tradename: Cytiva 28-4082-53
• storage condition: (20% Ehtanol); (20% Ethanol)
• parameter: 42 psi
• bed size: 7 mm × 25 mm
• bed volume: 1 mL
• column I.D.: 7 mm
• matrix: highly cross-linked agarose
• avg. part. size: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 2-12
• working range: 3-10
• capacity: ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
• suitability: suitable for bioprocess medium
• storage temp.: 2-8°C

Description

General description

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.

HiTrap^® MabSelect^™ columns are prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Application

Lab Purification using the MabSelect^™ Protein A tag for capture of antibodies.

Features and Benefits

• HiTrap^® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification.
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva

MabSelect is a trademark of Cytiva

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids