GE27-4810-01
Pezz 18 Expresion Vector
Cytiva 27-4810-01, pack of 1 ea
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About This Item
UNSPSC Code:
13111000
packaging
pack of 1 ea
manufacturer/tradename
Cytiva 27-4810-01
shipped in
dry ice
storage temp.
−20°C
General description
pEZZ 18 Protein A Gene Fusion Vector.
Application
The phagemid pEZZ 18 contains the Protein A signal sequence and two synthetic “Z” domains based on the “B” IgG binding domain of Protein A. Proteins are expressed as fusions with the “ZZ” peptide and secreted into the aqueous culture medium under the direction of the Protein A signal sequence. They are easily purified using IgG Sepharose™ 6 Fast FLow to which the “ZZ” domain binds tightly. Because of its folding properties, the 14 kDa “ZZ” peptide has little effect on folding of the fusion partner into a native conformation.
Expression is controlled by the lacUV5 and Protein A promoters and is not inducible. Elements of the Protein A gene provide the ATG and ribosome-binding sites. Stop codons must be provided by the insert.
M13 Universal Sequencing Primer can be used for both double-stranded and single-stranded sequencing. A protocol for production of single-stranded DNA is provided with the vector.
Inserts containing a stop codon will yield white colonies when grown on media containing X-gal.
Host(s). E. coli strains carrying a lac deletion but capable of a-complementation of lacZ′.
Expression is controlled by the lacUV5 and Protein A promoters and is not inducible. Elements of the Protein A gene provide the ATG and ribosome-binding sites. Stop codons must be provided by the insert.
M13 Universal Sequencing Primer can be used for both double-stranded and single-stranded sequencing. A protocol for production of single-stranded DNA is provided with the vector.
Inserts containing a stop codon will yield white colonies when grown on media containing X-gal.
Host(s). E. coli strains carrying a lac deletion but capable of a-complementation of lacZ′.
Features and Benefits
- For rapid expression of secreted fusion Proteins and their one-step purification using IgG Sepharose™ 6 Fast FLow
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sepharose is a trademark of Cytiva
Storage Class
13 - Non Combustible Solids
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