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G9768

Sigma-Aldrich

Guanosine 5′-triphosphate–Agarose

aqueous glycerol suspension

Synonym(s):

5′-GTP agarose

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1 ML
$117.00
5 ML
$359.79
25 ML
$1,071.00

$117.00

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All Photos(1)

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1 ML
$117.00
5 ML
$359.79
25 ML
$1,071.00

About This Item

MDL number:
MFCD00171655
UNSPSC Code:
41106500
NACRES:
NA.56

$117.00

Check Cart for Availability

biological source

bacterial (CORYNEBACTERIUM SP)
enzyme from Porcine brain
plant (Sea weed)
yeast

Quality Level

200

form

aqueous glycerol suspension

extent of labeling

1-5 μmol per mL

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

ribose hydroxyls

matrix spacer

11 atoms (adipic acid dihydrazide)

storage temp.

−20°C

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Application

Guanosine 5′-triphosphate-agarose (5′-GTP agarose) is an agarose conjugate in aqueous glycerol suspension used in affinity chromatography, for purification of guanosine-binding proteis

Physical form

Guanosine 5′-triphosphate–Agarose has been used:
  • in chromatographic column for the purification of a translocation stimulating factor[1]
  • in GTP-Agarose pull down assays to measure the levels of GTP-bound RHEB (Ras homolog enriched in brain)[2]
  • in GTP-Binding assay[3]
  • for the depletion of GTP binding proteins in cytosol of rat liver[4]
Suspension in 50% glycerol containing 0.25 M NaCl

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
SLCR1042
SLCQ2584
SLCL8415
SLCC0529
SLBT4518

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Possible role of both the alpha and beta gamma subunits of the heterotrimeric G protein, Gs, in transcytosis of the polymeric immunoglobulin receptor
Bomsel M and Mostov KE
The Journal of Biological Chemistry, 268, 25824-25835 (1993)
70K heat shock related proteins stimulate protein translocation into microsomes
Chirico WJ, et al.
Nature, 332, 805-805 (1988)
M Bomsel et al.
The Journal of biological chemistry, 268(34), 25824-25835 (1993-12-05)
In endosomes the polymeric immunoglobulin receptor (pIgR) is sorted into transcytotic vesicles. Transcytosis of the pIgR in polarized Madin-Darby canine kidney (MDCK) cells is regulated both by phosphorylation of Ser664 and by binding of ligand, dimeric IgA (dIgA). Mutation of
T E Allen et al.
Molecular and biochemical parasitology, 65(2), 233-245 (1994-06-01)
The hypoxanthine-guanine phosphoribosyltransferase (HGPRT) enzyme in Trypanosoma cruzi is a rational target for the treatment of Chagas disease. To evaluate the T. cruzi HGPRT in detail, the HGPRT gene (hgprt) was cloned from a genomic library of T. cruzi DNA
Somatic mutations in TSC1 and TSC2 cause focal cortical dysplasia
American Journal of Human Genetics, 100(3), 454-472 (2017)
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