PARD6G (Par-6 family cell polarity regulator γ) is a cell polarity protein exists in three homolgues: α, β, and γ form. It is composed of a PDZ domain and a CRIB-like motif.
Immunogen
synthetic peptide corresponding to amino acids 2-16 of human Par6γ conjugated to KLH. The corresponding sequence is identical in mouse and rat.
Application
Anti-Par6γ (N-terminal) antibody produced in rabbit is suitable for western blot analysis at a working dilution of 0.5-1μg/mL using lysates of HEK-293T cells over-expressing Par6γ.
Biochem/physiol Actions
The polarity complex, that is responsible for the establishment and maintenance of cell polarity, is composed of Par6, Par3 and atypical PKC isoforms (aPKC). These proteins have a key role in different cell polarization events that occur in vast range of species from worms to mammals.
Target description
Anti-Par6γ (N-terminal) recognizes human Par6γ (alsoknown as PAR6D). Mammalian genomes contain three Par6 homologues (designated in humans Par6 α, β and γ). All three Par6 proteins are structurally similar, but seem to have different tissue expression patterns and subcellular distribution.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Development (Cambridge, England), 122(10), 3133-3140 (1996-10-01)
The generation of asymmetry in the one-cell embryo of Caenorhabditis elegans is necessary to establish the anterior-posterior axis and to ensure the proper identity of early blastomeres. Maternal-effect lethal mutations with a partitioning defective phenotype (par) have identified several genes
Genes to cells : devoted to molecular & cellular mechanisms, 6(2), 107-119 (2001-03-22)
Asymmetric cell division in the Caenorhabditis elegans embryos requires products of par (partitioning defective) genes 1-6 and atypical protein kinase C (aPKC), whereas Cdc42 and Rac, members of the Rho family GTPases, play an essential role in cell polarity establishment
In many organisms, like Caenorhabditis elegans and Drosophila melanogaster, establishment of spatial patterns and definition of cell fate are driven by the segregation of determinants in response to spatial cues, as early as oogenesis or fertilization. In these organisms, a
Biochimica et biophysica acta, 1778(3), 614-630 (2007-11-17)
The formation of functional epithelial tissues involves the coordinated action of several protein complexes, which together produce a cell polarity axis and develop cell-cell junctions. During the last decade, the notion of polarity complexes emerged as the result of genetic
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