G9670

Monoclonal Anti-O-Glycosylated Neurofilament-M antibody produced in mouse

~2 mg/mL, clone NL6, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-O-glycosylated NF-M
• MDL number: MFCD09265427
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: NL6, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~160 kDa
• species reactivity: human, rat
• should not react with: bovine, mouse
• packaging: antibody small pack of 25 μL
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: 0.5-1 μg/mL using human NT2 cell extracts
• UniProt accession no.: P07197
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NEFM(4741) ; rat ... Nefm(24588)

Description

General description

Intermediate filaments (IFs) have a diameter of 8-10nm and differ from microtubules and microfilaments significantly. Neurofilaments (NFs) are a type of IFs found mainly in cells or tissues of neuronal origin. NFs are composed of three major proteins, NF-L, NF-M and NF-H, having a molar mass of 68kDa, 160kDa, and 200kDa, respectively.

Monoclonal Anti-O-Glycosylated Neurofilament-M (mouse IgG2a isotype) is derived from the hybridoma NL6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a protein fraction enriched for components of the human neuronal cytoskeleton. Neurofilament medium (NEFM) gene encodes neurofilament medium polypeptide which constitutes to cytoskeleton of mature neurons. The gene in human chromosome is localized on 8p21.

Immunogen

protein fraction enriched for components of the human neuronal cytoskeleton from cell lines NT2-N and SK-N-BE(2). The epitope is localized in the carboxy-terminal tail domain of NF-M.

Application

Monoclonal Anti-O-Glycosylated Neurofilament-M antibody produced in mouse has been used in:

• immunohistochemical methods
• immunoblotting
• immunocytochemistry

Biochem/physiol Actions

Neurofilament medium (NEFM) is essential for radial growth of axon which includes the diameter of the axon as well as the myelination. The C-terminus of NFEM gene has 7 repeats of KSP (lysine-serine-proline) domain and phosphorylation of which is crucial for regulating the radial growth of axon.

The NF proteins are synthesized in the neuronal perikarya before assemby to form the filaments. These filaments are then transported within the axons towards the synaptic terminals. The function of these NF polypeptides is regulated by phosphorylation, which influences their interaction with cytoplasmic organelles. The head region of NF-L and NF-M contain sites modified by O-linked GlcNAc. Down-regulation of NF-M O-GlcNAcylation and increased phosphorylation of NF-M is observed in Alzheimer disease. This antibody recognizes specifically an O-glycosylated epitope in the projection domain of NF-M. In a rat model for amyotrophic lateral sclerosis (ALS), this specific modification is strongly decreased compared with wild-type animals.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)